Group Title: AREC-A research report - Agricultural Research and Education Center-Apopka ; RH-86-13
Title: Comparison of BA, Kinetin and 2iP for inducing basal shoot production in Spathiphyllum 'Bennett'
CITATION THUMBNAILS PAGE IMAGE ZOOMABLE
Full Citation
STANDARD VIEW MARC VIEW
Permanent Link: http://ufdc.ufl.edu/UF00065911/00001
 Material Information
Title: Comparison of BA, Kinetin and 2iP for inducing basal shoot production in Spathiphyllum 'Bennett'
Series Title: AREC-Apopka research report
Physical Description: 4 p. : ; 28 cm.
Language: English
Creator: Henny, R. J
Fooshee, W. C ( William C. ), 1951-
Agricultural Research and Education Center (Apopka, Fla.)
Publisher: University of Florida, IFAS, Agricultural Research and Education Center-Apopka
Place of Publication: Apopka FL
Publication Date: 1986
 Subjects
Subject: Spathiphyllum -- Growth -- Florida   ( lcsh )
Shoots (Botany) -- Florida   ( lcsh )
Growth regulators -- Testing   ( lcsh )
Genre: government publication (state, provincial, terriorial, dependent)   ( marcgt )
non-fiction   ( marcgt )
 Notes
Statement of Responsibility: R.J. Henny and W.C. Fooshee.
General Note: Caption title.
 Record Information
Bibliographic ID: UF00065911
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 70667522

Full Text





HISTORIC NOTE


The publications in this collection do
not reflect current scientific knowledge
or recommendations. These texts
represent the historic publishing
record of the Institute for Food and
Agricultural Sciences and should be
used only to trace the historic work of
the Institute and its staff. Current IFAS
research may be found on the
Electronic Data Information Source
(EDIS)

site maintained by the Florida
Cooperative Extension Service.






Copyright 2005, Board of Trustees, University
of Florida








--.-\b
Comparison of BA, Kinetin and 2iP for Inducing Basal Shoot
Production in Spathiphyllum 'Bennett'

1 Cen.tr! Science
R. J. Henny and W. C. Fooshee L ibrrv
University of Florida, IFAS
Agricultural Research and Education Center Apokti A 1987
AREC-Apopka Research Report RH-86-13

BA (N -benzyladenine) applied as a soil drench has been shoin 'to or'g
stimulate basal shoot production in Spathiphyllum (1) 7-This--study-waL.s _
conducted to test efficacy of BA compared to two other cytokinins, Kinetin
(6-furfurylaminopurine) and 2iP (N -[A -iscpentenyl] adenine).

Tissue-culture derived cuttings of Spathiphyllum 'Bennett' were rooted
and established in 2.5 cm plastic cells in a medium of Vqrgro (2:1:1
Canadian peat:perlite:vermiculite) amended with 0.9 kg/m Micromax
(micronutrient source). Liners were then potted to 10 cm pots in the same
medium and fertilized with 2.5 gms Osmocote (19-6-12) per pot. Two weeks
after potting, plants were drenched with a 10 ml solution of BA, Kinetin or
2iP at 125, 250 or 500 mg/L poured on the base of each plant. Solutions
were prepared using the guidelines in Table 1. There were 10 pots
containing a single plant for each treatment plus 10 pots treated with
deionized water (control). Each pot was considered an experimental unit.
The number of visible basal shoots was recorded at 4-week intervals for 20
weeks. At the end of this period plant height and fresh weight of the main
central shoot (minus roots) plus fresh weight of basal shoots (minus roots)
were also recorded. This experiment was conducted from 9-26-85 to 2-12-86
(fall test) and repeated from 12-4-85 to 4-25-86 (winter test).

In the first experiment, BA was effective in stimulating basal shoot
production at all three concentrations, Kinetin was moderately effective at
the 250 and 500 mg/L rates whereas 2iP was ineffective regardless of rate
(Table 2). BA at 250 and 500 mg/L more than doubled the number of basal
shoots (10.1 and 12.2 respectively) compared to the controls (4.9). Fresh
weight of the basal shoots was greater than the main shoot at the two
highest BA levels. Fresh weight of the main shoot was significantly
smaller at the two highest BA rates. There was no significant difference
in total fresh weight among any treatments. Similar results from BA
treatment had been observed in earlier studies (1).

Results from the second experiment were similar to the first test.
Kinetin and 2iP did not increase basal shoot production while BA did (Table
3). BA applied at 500 mg/L was the only treatment which doubled the basal
shoot number (8.2) compared to controls (3.9). Fresh weight and height of
the main stem decreased as the BA level increased. Fresh weight of basal
shoots nearly equaled that of the main shoot at the 500 mg/L BA rate.
Although more variable than the first test, again there was no significant


Associate Professor and Biological Scientist III, Plant Genetics,
Agricultural Research and Education Center, 2807 Binion Road, Apopka, FL
32703, respectively.









difference in total fresh weight among any treatments.

A large difference in total growth (as determined by fresh weight
measurements) was observed between the two experiments. Plants in the
winter test produced only 53% as much fresh weight as those in the fall
study. Overall height of the main shoot during the winter test was 86% of
the fall test. These differences in growth may be attributed to cooler
temperatures (slower growth) and lower light (taller plants) levels during
the winter season. The smaller increase in basal shoot number resulting
from BA treatment may also be due to less than optimum growing conditions.
Further seasonal tests would be required to verify this hypothesis.

Results from this study can be summarized as follows:

1. BA at 250 or 500 mg/L applied as a soil drench is effective in
stimulating basal shoot development in Spathiphyllum 'Bennett'
while Kinetin and 2iP are not;

2. The increase in basal shoot production occurs at expense of main
shoot as growth is redirected to laterals;

3. Total growth (fresh weight) is unaffected by BA treatment,

4. Plants treated in the fall responded better to BA-treatment than
those treated in the winter and may be related to growth
conditions;

5. A BA level of 500 mg/L is recommended for most consistent results
regardless of season;

6. The cost of BA-treatment (including labor) using these methods was
calculated to be 0.9 cents per plant. See Table 1 for source and
cost of BA;

7. The increase in basal shoot production due to BA-treatment may
allow growers to use one plant per pot rather than multiples
thereby making it an economically feasible growing method.

Literature Cited

1. Henny, R. J. and W. C. Fooshee. 1985. Induction of basal shoots in
Spathiphyllum 'Tasson' following treatment with BA. HortScience
20:715-717.


-2-




Table 1. Procedure for preparing 10 liters of a 500 mg/L BA solution.

1. Add 500 ml of water to large container with at least a 10 liter capacity;

2. Add 10 milliliters of full strength Muratic acid to the water;

3. Add 3-4 drops wetting agent (stir to blend);

*4. Add 5 grams BA crystals to the above solution;

5. Stir until all the BA crystals are dissolved (this may take 3-5 minutes
of constant stirring);

6. Once all the crystals have been dissolved add enough water to bring the
final volume to 10 liters;

7. This produces -10 liters of a 500 mg/L BA solution which will remain
stable for several days.

*BA Source; Polaroid Corporation
238 S. Main Street
Assonet, Mass. 02702
(617) 675-0808
Cost. $315.00 per Kilogram (smallest amount purchasable).




Table 2. Mean number of visible basal shoots, height of main shoot plus fresh
weight of main shoot (minus roots) and basal shoots (minus roots) and total
fresh weight of main plus basal shoots 20 weeks after treatment of
Spathiphyllum 'Bennett' with BA, Kinetin or 2iP applied as a soil drench.

SNumnber H trmafn'' Fresh t. fees
Concn. basal shoot main shoot basal shoots Total fresh
Treatmentz mg/L shoots (cm) (gms) (gms) wt. (gms)

Control 0 4.9 33.4 40.7 24.4 65.1

BA- 125 --- 1 36.2 37.6 37.0 74-6--
250 10.1 33.1 27.9 36.4 64.2
500 12.2 31.0 26.7 38.5 65.2

Kmnetffn 125----- --4,8 36.3 377T.4 -- ." -.T. 55.3
250 6.4 36.8 42.0 27.8 69.8
500 7.3 34.7 39.5 27.8 67.2

21P 125 5.4 37.0 43.9 22.9 66.8
250 5.0 36.6 40.6 22.7 63.3
500 4.9 34.8 36.3 29.6 65.9


Significant effectsy

Treatment ** ** ** ** NS
Conc. NS NS
Treatment x Conc. NS NS NS


ZPlants treated with
10 replications per

YSignificance of the
nonsignificant.


10 ml of solution per 10 cm pot on September 26, 1985.
treatment.

F value denoted as follows: ** = 1%, = 5% and NS =


-3-









Table 3. Mean number of visible basal shoots, height of main shoot plus fresh
weight of main shoot (minus roots) and basal shoots (minus roots) and total
fresh weight of main plus basal shoots 20 weeks after treatment of
Spathiphyllum 'Bennett' with BA, Kinetin or 2iP applied as a soil drench.


Number Ht. main


basal shoot
shoots (cm)


3.9


5.3
6.3
8.2


30.4


31.4
28.2
27.1


Fresh wt. Fresh wt.
main shoot basal shoots
(gms) (gms)


27.7


27.1
20.8
17.8


10.8
9.9
17.4


Kinetin 125 4.5 34.4 34.5 9.4 43.9
250 3.4 29.8 25.8 5.6 31.5
500 3.7 30.4 24.9 4.1 29.0

2iP 125 2.7 31.8 28.5 4.8 33.3


250
500


4.5
3.2


32.1
27.5


30.9
23.7


9.1
5.9


40.7
29.6


Significant effectsy


Treatment
Conc.
Treatment x Conc.

ZPlants treated with
10 replications per

Significance of the
nonsignificant.


10 ml of solution per 10 cm pot on December 4, 1985.
treatment.

F value denoted as follows. ** = 1%, = 5% and NS =


- 4-


Treatmentz


Conc.
mq/L


Control


125
250
500


Total
fresh wt.
(gms)


35.4


38.5
30.7
35.2


----- --Lgms)


-- ------




University of Florida Home Page
© 2004 - 2010 University of Florida George A. Smathers Libraries.
All rights reserved.

Acceptable Use, Copyright, and Disclaimer Statement
Last updated October 10, 2010 - - mvs