Group Title: Research report - Ft. Pierce Agricultural Research and Education Center ; RL-82-1
Title: Identification of Macroptilium Atropurpureum accessions resistant to Uromyces Appendiculatus
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 Material Information
Title: Identification of Macroptilium Atropurpureum accessions resistant to Uromyces Appendiculatus
Series Title: Ft. Pierce ARC research report
Physical Description: 3, 4 leaves : ; 28 cm.
Language: English
Creator: Sonoda, Ronald M
University of Florida -- Agricultural Research Center
Publisher: University of Florida, Insititute of Food and Agricultural Sciences, Agricultural Research Center
Place of Publication: Fort Pierce Fla
Publication Date: [1982]
 Subjects
Subject: Legumes -- Diseases and pests -- Florida   ( lcsh )
Genre: bibliography   ( marcgt )
non-fiction   ( marcgt )
 Notes
Bibliography: Includes bibliographical references (leaf 3).
Statement of Responsibility: R.M. Sonoda and A.E. Kretschmer, Jr.
General Note: Caption title.
General Note: "June 1982."
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Bibliographic ID: UF00055979
Volume ID: VID00001
Source Institution: University of Florida
Rights Management: All rights reserved by the source institution and holding location.
Resource Identifier: oclc - 67290192

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Ft. Pierce ARC.Research Report RL-82-1 June 1982


IDENTIFICATION OF MACROPTILIUM ATROPURPUREUM ACCESSIONS
RESISTANT TO UROMYCES APPENDICULATUS.

R. M. Sonoda1 and A. E. Kretschmer, Jr.1


ABSTRACT

Twenty of 104 Macroptilium atropurpureum accessions tested were
highly resistant to Uromyces appendiculatus when inoculated with
uredospore suspensions. There were 18 accessions that were judged
intermediate in response. Eighteen of the 71 accessions from Mexico
were resistant. Two of three accessions from Colombia were resistant.
The resistant accessions consisted of broad leaflet types similar in
morphology to the cultivar 'Siratro' and narrow leaflet types estimated
to be less productive of shoot matter than the broad leaflet types.



Rust incited by Uromyces appendiculatus Fries on Macroptilium
atropurpureum (DC) Urb. can be severe in the spring and fall in south
Florida. The disease can stunt growth of the host. Symptoms of the
disease, pustules on leaves and stems, are usually absent from foliage
produced in the summer.

Several accessions of M. atropurpureum collected in Mexico were
previously found to be resistant U. appendiculatus (1). Only one of
these resistant accessions, IRFL 3440, was similar to Siratro in --
agronomic characteristics (1). Results of screening new accessions
of M. atropurpureum at the Agricultural-Research Center, Fort Pierce,
Florida against U. appendiculatus are reported.




Professors, University of Florida, Institute of Food and Agricultural
Sciences, Agricultural Research Center, Fort Pierce.











MATERIALS AND METHODS

M. atropurpureum accessions were obtained from several locations.
An accession each from Venezuela, Belize, Brazil, three accessions
from Colombia, and 78 accessions'collected from Mexico, 67 of which
were obtained from Dr. R. Reid, Townsville Australia were tested.
Also included in the test were four accessions from Brazil that-were
previously screened (1) and 29 accessions from Mexico that were pre-
viously screened (2).

Scarified and germinated seed were transferred to 1:1 horticultural
vermiculite:sphagnum peat moss (wt/wt) and dusted with cowpeaa type'
inoculant. The germinated seeds were placed one seed to a-cell in 72-
celled 'Speedling' styrofoam trays in a randomized complete block design
of five replications. A suspension of U. appendiculatus was prepared by
suspending and shaking rust-infected leaves from different accessions
of M. atropurpureum in distilled water. The suspension was centrifuged
at 3000 rpm with an IEC CS centrifuge and uredospores resuspended in
distilled water. A suspension of about 5 x 104 uredospores per ml was
sprayed on the.lower surface -of the first true leaf when the second
true leaf on most plants was about one-half expanded. The inoculated
plants in speedling trays were placed in large black polyethylene bags
and incubated for 24 hr in the laboratory (25-280C). The plants in
trays were removed from the polyethylene bags and placed on an outside
bench in the shadow of some large trees. Disease severity ratings were
made 13 days after inoculation on a;0 (no disease) to 3 (high incidence
, of rust pustules) scales.

RESULTS-AND DISCUSSION

Of the 104 M. atropurpureum accessions screened, lesions were ab-
sent from 20. These accessions are listed on Table:l. Three-of these
accessions, -IRFL 3350, 3440, and 3531 were previously reported to be
resistant (2). Eighteen-accessions (Table 2) were intermediate in re-
sistance (Disease severity rating ranging from 0.2 to 1.4). The others.
(Table .3) were susceptible.
Resistance to the U. appendiculatus affecting Siratro and other
M. atropurpureum in south Florida occurs in many of the accessions from-
Mexico. -The two resistant accessions from Colombia indicate that sources
of resistance-may be in other'areas in the natural range of the species.
R. J. Jones (personal communication) indicated that accession. IRFL 581.7
(CQ 1382) was resistant to the rust in Australia.- In our test, four of
five plants of this accession were resistant.. Further study is being
conducted on this accession. -Field studies will be made with the resistant
accessions to determine if they are as productive as Siratro.








-3-


LITERATURE CITED


1) Sonoda, R. M. 1976. Reaction to Macroptillium atropurpureum and
related species to three diseases in Florida. Tropical Grasslands,
10:61-63.

2) Sonoda, R. M. and A. E. Kretschmer, Jr. 1982. Sources of resistance
of Macroptilium atropurpureum to rust. Soil and Crop Sci. Soc. Fla.
Proc. 41:(In Press)











Table 1. Macroptilium atropurpureum accessions on which no pustules
were formed when inoculated with Uromyces appendiculatus
attacking cultivar 'Siratro'.



Australian Country
IRFL # or other # of
CPI # origin


2937 Colombia
2938 -- -- Colombia
3350 87486 Mex 343 Mexico
3440 -- -- Mexico
3531 -- -- Mexico
3586 84578 Mex 038 Mexico
3599 84999 Mex 095 Mexico
5077 -- -- Mexico
5143 90915 Mex 901 Mexico
5190 91101 Mex 939 Mexico
5740 87849 Mex 462 Mexico
5749 90463 Mex 678 Mexico
5754 90822 Mex 808 Mexico
5755 90845 Mex 831 Mexico
5756 90847 Mex 833 Mexico
5757 90850 Mex 836 Mexico
5758 91106 Mex 944 Mexico
5759 91157 Mex-995 Mexico
5767 -'" 91485-- Mex 1182'- Mexico
5768 91490 Mex 1187 Mexico



University of Florida, Agricultural Research Center, Fort Pierce
accession number

Australian accession #.

Mexican accession #.











Table 2. Macroptilium atropurpureum accessions on which few pustules
were formed when inoculated with Uromyces appendiculatus
attacking cultivar 'Siratro'l.


IRFL #2


3356
3462
3524
3568
5129
5142
5729
5730
5731
5734
5739
5742
5746
5747
5748
5750
5753
5761


CPI #3


87488



90486
90905
84988
84989
84993
85852
87846
87878
90337
90338
90455
90470
90821
91348


MEX #4


701
891
084
085
089
187
459
491
560
561
670
685
807
1128


DSI5


0.6
1.4
1.0
1.2
1.4
0.4
1.4
0.2
0.4
0.6
1.2
1.2
1.2
1.0
1.2
1.0
0.4
0.8


1All accessions on table collected from Mexico by
or R. Reid and A. E. Kretschmer, Jr., University
Research Center, Fort Pierce, FL.


Reid, CSRIO, Australia
Florida, Agricultural


2University of Florida, Agricultural Research Center, Fort Pierce accession
number.
3 -
Australian accession number.

Mexican accession number.

DSI Disease severity index 0 = no disease to 3 = high incidence of rust
pustules.








Table 3. Macroptilium atropurpureum accessions highly susceptible to
Uromyces appendiculatus.



IRFL PI 2 Australiaother DSI5
or
CPI #


483 -- Siratro -- 2.4
911 322576 CQ 364 IRI 1390 2.8
913 322578 18556 IRI 1392 2.8
916 322579 -- IRI 1553 1.8
917 -- 18556 IRI 2543 2.3
1055 -- -- 2.6
1502 -- -- 1.8
2117 414393 -- Y 103 2.4
2118 414394 -- Y 50 2.8
2206 414394 -- CF 3-2 2.8
2207 -- CF 360 2.3
2209 -- CF 386 3.0
2211 -- -- CF 409 3.0
2212 -- CF 411 2.8
2213 -- CF 442 2.6
2214 -- CF 568 2.6
3146 -- IRI 3316 2.8
3382 -- 87502 MEX 359 2.5
3383 -- 87504 MEX 361 3.0
3420 .- -- -- 2.6
3426 -- -- 2.6
3436 -- -- -- 2.2
3457 -- -- 2.5
3508 -- -- -- 2.6
3521 -- -- 2.4
3527" -- -- 1.6
3555 -- -- 1.8
3561 -- 2.6
3564 -- -- 2.0
3580- -- -- 1.8
3600 -- 85002 MEX 098 2.0
3620 -- 85900 MEX 233 2.4
3624 -- 85919 MEX 252 2.8
3625 -- 85924 MEX 257 1.8
3636 -- IPI 985 2.8
5132 -- 90775 MEX 761 1.8
5544 -- CF 706 3.0
5545 -- CF 741 2.5
5546 -- CF 572 1.8
5547 -- CF 755 2.4
5548 -- CF 217 3.0
5549 -- CF 223 2.6
5550 -- CF 713 1.8
5551 -- -- CF 439 2.5
5552 -- CF 703 2.8














Table 3. (continued)


Australian
or
CPI #


84995
87528
87532
87546
87807
87869
87880
90335
90748
90776
91193
91437
91445
91447
91463
91468
91498


5553
5554
5732
5735
5736
5737
5738
5741
5743
5744
5751
5752
5760
5762
5763
5764
5765
5766
5769
5815
5816


University of
accession #.


Florida, Agricultural Research Center, Fort Pierce


2United States Department of Agriculture plant introduction number.

Australian accession #.

Other #'s IRI Brazil
Y Belize
CF Mexico
MEX Mexico
IPI Ipinia, Ecuador

5Disease Severity Index: 0 = no disease to 3 = high incidence of
rust pustules.


IRFL #


PI # 2


other #4


DSI5


Siratro


CF
CF
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX
MEX


570
599
091
385
389
403
420
482
493
558
734
762
1030
1134
1142
1144
1160
11657
1195


2.8
2.4
1.8
2.8
3.0
2.8
2.8
2.2
1.6
2.2
2.5
2.8
1.8
2.8
2.8
2.0
2.4
3.0
2.8
2.8
2.6


I I w




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