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The Alachua County Historic Trust: Matheson Museum, Inc. houses a library, archives and museum with an extensive collection of Florida and local history books and periodicals, and a growing collection of local historical maps, photographs, papers, and museum objects. The digital collections of the Matheson Museum represent a fraction of these holdings.

The Museum is funded in part by grants from the City of Gainesville, Department of Cultural Affairs, and the Historical Museum Grants-in-Aid Program through the Bureau of Historical Museums, Division of Historical Resources, Florida Department of State, Secretary of State. Digitization is funded through a variety of sources including local partnerships and grants, including a grant from the Institute for Museum and Library Services.