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Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen
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Permanent Link: http://ufdc.ufl.edu/IR00001640/00001
 Material Information
Title: Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen
Physical Description: Fact Sheet
Creator: Hansen, Gary R.
Publisher: University of Florida Cooperative Extension Service, Institute of Food and Agriculture Sciences, EDIS
Place of Publication: Gainesville, Fla.
Publication Date: 2006
 Notes
Acquisition: Collected for University of Florida's Institutional Repository by the UFIR Self-Submittal tool. Submitted by Melanie Mercer.
Publication Status: Published
General Note: "Original publication date April, 2006"
 Record Information
Source Institution: University of Florida Institutional Repository
Holding Location: University of Florida
Rights Management: All rights reserved by the submitter.
System ID: IR00001640:00001

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AN163 Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen1 Gary R. Hansen2 The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations. U.S. Department of Agriculture, Cooperative Extension Service, University of Florida, IFAS, Florida A. & M. University Cooperative Extension Program, and Boards of County Commissioners Cooperating. Larry Arrington, Dean Introduction Sorting Sperm Cells

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Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 2 Economic Implications Bostaurus Bos indicus Bos taurus Bos indicus Challenges using sexed semen. Select Sires has conducted Using Sexed Semen Insure that semen is stored at proper

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Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 3 Thaw straws according to procedures Insure that AI equipment is clean and sanitary. Warm equipment (sheaths, guns, paper Avoid exposing semen to direct sunlight or Do not use lulubricants that are spermicidal. Use highly qualified technicians when using Insure animals are in good body condition Inseminate cattle twelve hours (AM/PM rule) Breed only those animals that display heat if Figure 1 Diagram Figure 1. Diagram illustrating sperm sexing procedures using a flow cytometer/cell sorter to measure DNA content. Credits: Adapted from Embryo Transfer in Dairy Cattle Copyright by W. D Hoards and & Sons Company (used by permission). Figure 2. MoFlo flow cytometer/cell sorter

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Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 4 Figure 3. Close up of interrogation tower Figure 4. Continuously charged electric plates.

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Select the Sex of Your Next Calf Prior to Mating: Using Sexed Semen 5 Table 1. Pregnancy rates in heifers inseminated with low dose fresh or frozen sexed semen and conventional frozen semen. No. of Heifers Cells/dose % pregnant % female Sexed liquid semen 37 .5 Million 30% 100% Sexed frozen semen 35 1 Million 51% 94% Frozen control semen 37 40 Million 73% 59% Table 2. Conception rates using sexed semen for 3 Holstein sires. Sire ID Number of Services Conception rate 7HO6329 645 42 7HO6454 730 45 7HO6685 874 46 Non-Sexed --61