Adapting Algae to Ammonia Rich Environments for Wastewater Treatment

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Adapting Algae to Ammonia Rich Environments for Wastewater Treatment
Lane, James Jacob Underhill
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Cyanothece algae were adapted to have a tolerance of summa ammonia environments (about 1200 mg/L) for wastewater treatment. The effects of keeping algae of the same generation in containers with various levels of ammonia content was studied. As that generation was given ample time to adapt to each ammonia-rich environment, the seed from the algae able to survive in the highest concentration was then used as the next generation and was exposed to higher ammonia concentrations (this process is explained later in the paper). The algae and its housing was tested daily to ensure the conditions of each vial remained constant throughout the experiment. Salinity in each jar was kept to 30 g/L, pH was kept at 6.8-7.8. Optical density (ODE) and dry weights were taken to measure algae growth within each container. It was determined in this experiment that the algae could be adapted to an ammonia level of about 1200 mg/L. This experiment was important to the ultimate goal of using the algae for wastewater treatment in that it could be used as a sustainable source in areas where they would normally not be able to survive. ( en )
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Awarded Bachelor of Science in Chemical Engineering, summa cum laude, on May 8, 2018. Major: Chemical Engineering
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College or School: College of Engineering
General Note:
Advisor: Spyros Svoronos. Advisor Department or School: Chemical Engineering

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University of Florida
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University of Florida
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Copyright James Jacob Underhill Lane. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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