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Effect of Kinase Inhibitors on DAXX and FASN Localization in Triple-Negative Breast Cancer Cells
Clees, Jennifer Michelle
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This thesis outlines efforts to determine the effect of various kinase inhibitors on DAXX and FASN localization in triple-negative breast cancer. Triple-negative breast cancer (TNBC) is characterized by its lack of estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptor 2 (HER2) amplification. Patients with this aggressive, invasive form of breast cancer (BC) have a nearly one in three chance of relapse within five years of chemotherapy. The poor prognosis associated with TNBC acts as an impetus for researchers to discover better, more targeted treatment options. This study has used the TNBC cell line MDA-MB-231, a p53 mutant line, to investigate the effects of various novel small-molecule kinase inhibitors. Kinases are involved in phosphorylating a large variety of molecules active in signaling transduction pathways, the cell cycle, and apoptosis, or programmed cell death. In particular, inhibitors to certain kinases act on the cellular localization of two proteins of interest: death domain-associated protein 6 (DAXX) and fatty acid synthase (FASN), and as a result, the proliferative abilities of TNBCs. DAXX functions as a transcription repressor in the nucleus and is involved in apoptosis, while the enzyme FASN catalyzes a terminal step in de novo lipogenesis. ( en )
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Awarded Bachelor of Science, summa cum laude, on May 8, 2018. Major: Chemistry. Emphasis/Concentration: Biochemistry
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College or School: College of Liberal Arts and Sciences
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Advisor: Daiqing Liao. Advisor Department or School: Deptartment of Anatomy and Cell Biology

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University of Florida
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University of Florida
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Copyright Jennifer Michelle Clees. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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