Citation
Prototyping an Open Source Single Plane Illumination Microscope for 3D Histology

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Title:
Prototyping an Open Source Single Plane Illumination Microscope for 3D Histology
Creator:
Berko, Joshua Alexander
Publication Date:
Language:
English

Notes

Abstract:
Histology is the study of microscopic structures of tissues. By analyzing the tissue samples, diseases and injuries can be studied. Typically, sectioned, 2D tissue samples are used for histological evaluation. However, there are shortcomings of 2D histologic methods, including the work intensive process of manual sectioning, specimen destruction, and limitations of two-dimensional analysis of tissues [1]. It is worth noting, a technique called serial histological sectioning (SHS) utilizes z-stacking digital images of slides to reconstruct a 3D image. This provides a unique perspective of the specimen and, potentially, deeper insight into the tissue anatomy. However, manually sectioning slides is still time consuming, and the process of sectioning and creating the reconstruction is still destructive to the sample. Single plane illumination microscopy (SPIM) provides an alternative method of generating histological images and 3D reconstructions. SPIM uses a single plane of laser to illuminate individual sections of a specimen, which can be reconstructed into 3D images via z-stacking. SPIM can provide faster imaging speeds, minimal photo-inducing tissue damage, and sub-cellular resolution, but commercially available microscopes are often prohibitively expensive [2]. As a result, the OpenSPIM platform was created as an open source library for constructing a SPIM with peer support, for a fraction of commercial price. In this work, steps toward prototyping a SPIM based on the OpenSPIM platform were taken, with the ultimate goal of demonstrating the usefulness of 3D histology when analyzing models of OA. ( en )
General Note:
Awarded Bachelor of Science in Biomedical Engineering, magna cum laude, on May 8, 2018. Major: Biomedical Engineering
General Note:
College or School: College of Engineering
General Note:
Advisor: Kyle Allen, Ph.D.. Advisor Department or School: Biomedical Engineering

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Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
Copyright Joshua Alexander Berko. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

UFDC Membership

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UF Undergraduate Honors Theses

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