Citation
Antibiotic Activity Of Propolis Available On The Market

Material Information

Title:
Antibiotic Activity Of Propolis Available On The Market
Series Title:
19th Annual Undergraduate Research Symposium
Creator:
Suguieda, Mariana
Lanham, Ashleigh-Marie
Desroches, Eric
Language:
English
Physical Description:
Undetermined

Subjects

Subjects / Keywords:
Center for Undergraduate Research
Center for Undergraduate Research
Genre:
Conference papers and proceedings
Poster

Notes

Abstract:
With the increase of antibiotic resistance in many pathogens, it is important to research and develop alternative methods of killing harmful microbes. Propolis, commonly known as “bee glue,” is a substance composed of resins that bees acquire from trees and flowers to support and protect their hive from pathogens. Current research suggests that propolis has anti-bacterial, anti-fungal, and anti-oxidant properties. This project focused on the anti-bacterial properties of three brands of propolis available on the market (Y.S Eco Bee Farm, Pon Lee, and Apiario) against four bacteria: E. coli, S. aureus, P. aeruginosa, and L. acidophilus. We predicted that the three different brands of propolis would show activity against all four bacteria but stronger activity against the gram-positive bacteria based on previous research. Using the Kirby-Bauer disc diffusion method for antibiotic testing, each brand was tested twice against each strain of bacteria. The results suggested that Pon Lee was the most effective brand tested as it showed activity against all bacteria tested. All brands showed more activity against the gram-positive bacteria. With more research, propolis has the potential to supplement or even reduce antibiotic use in both medical and agricultural fields. ( en )
General Note:
Research authors: Mariana Suguieda, Eric Desroches, Ashleigh-Marie Lanham, Victoria Williams, Jennifer Diaz, Andrew Murphy - University of Florida
General Note:
Faculty Mentor: With the increase of antibiotic resistance in many pathogens, it is important to research and develop alternative methods of killing harmful microbes. Propolis, commonly known as “bee glue,” is a substance composed of resins that bees acquire from trees and flowers to support and protect their hive from pathogens. Current research suggests that propolis has anti-bacterial, anti-fungal, and anti-oxidant properties. This project focused on the anti-bacterial properties of three brands of propolis available on the market (Y.S Eco Bee Farm, Pon Lee, and Apiario) against four bacteria: E. coli, S. aureus, P. aeruginosa, and L. acidophilus. We predicted that the three different brands of propolis would show activity against all four bacteria but stronger activity against the gram-positive bacteria based on previous research. Using the Kirby-Bauer disc diffusion method for antibiotic testing, each brand was tested twice against each strain of bacteria. The results suggested that Pon Lee was the most effective brand tested as it showed activity against all bacteria tested. All brands showed more activity against the gram-positive bacteria. With more research, propolis has the potential to supplement or even reduce antibiotic use in both medical and agricultural fields. - Center for Undergraduate Research,

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University of Florida
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Copyright Mariana Suguieda. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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introduction propolis antibiotic activity mariana suguieda, ashleigh marie-lanham, eric desroches, jennifer diaz, andrew murphy, and victoria williams What is Propolis? Results methods conclusions 14 16.5 0 0 14 17 11.5 11 12.5 20.5 11 0 S. AUREUS L. ACIDOPHILUS E. COLI P. AERUGINOSA ZONE OF INHIBITION (MM) BACTERIA ANTIBIOTIC ACTIVITY OF PROPOLIS Brand 1 Brand 2 Brand 3 With the increase of antibiotic resistence in many pathogens, it is important to research and develop alternative methods of combatting harmful microbes. Propolis is a substance made by bees for structural support and protection from pathogens. is project will focus on the anti-bacterial properties of propolis available on the market against four bacteria: E. coli, S. aureus, P. aeruginosa, and L. acidophilus. We predict that the three propolis brands available on the market will show activity against these four bacteria but will show stronger activity against the gram-positive bacteria based on previous research. e objective is to determine if the propolis that is available to consumers is eective against these microbes. Propolis is a resinous substance known as bee glue that serves as a protective barri er for bee hives. Bees make propolis from the leaves and owers they land on, making the compo sition of propolis variable by hive, season, and geographic location. Brand 1 Y.S Eco Farms Brand 2 pon lee Brand 3 apiario Using the Kirby-Bauer antibiotic susceptibility testing method, Mueller-Hinton plates were divided into three quadrants for each propolis and inoculated with S. aureus, L. acidophilus, E. coli, and P. aeruginosa individually using aseptic technique. One disc was saturated with one brand of propolis and then placed onto the agar in the appropriate quadrant. is was done with all three brands across the four plates with dierent bacteria. ese plates were then incubated at 37 degrees Celsius for 48 hours. Lastly, the zones of inhibtion were measured. Two trials were conducted. X4 step 1: inoculate one bacterium step 2: place three saturated discs of propolis MHA Plates e table above represents the zones of inhibition mea sured aer 48 hours in the incubator. Brand 1 and 2 performed the same against S. aureus while Brand 3 performed slightly worse. Brand 3 performed best against L. acidophilus Brand 2 worked the best against E. coli while Brand 1 did not work at all. Brand 2 was the only brand to work against P. aeruginosa and thus was the only brand that worked against all four strains. All three brands showed more antibiotic activity against Gram-positive bacteria than Gram-negative. step 3: Incubate, then Measure zones of inhibtion All three brands had notable zones of inhibition against of S. aureus and L. acidophilus rather than E. coli and P. aeruginosa suggesting that the prop olis is more eective against Gram-positive bacte ria than Gram-negative bacteria as our hypothesis stated. Brand 2 (Pon Lee) was the only brand that showed antibiotic activity against all four bacte ria so we concluded it would be the most eective propolis tested. Although not as eective as many modern med icines, propolis can be used as a natural medicine to supplement conventional antibiotics. references 1. Bankova, V. (2005). Chemical diversity of propolis and the problem of standardization.Journal of Ethnopharmacology,100(1-2), 114-117. doi:10.1016/j.jep.2005.05.004 2. Bankova, V. S., Castro, S. L., & Marcucci, M. C. (2000). Propolis: recent advances in chemistry and plant origin. Apidologie, 31(1), 3-15. doi:10.1051/apido:2000102 3. Cushnie, T. T., & Lamb, A. J. (2006). Errata for Antimicrobial activity of avonoids [Int. J. Antimicrob. Agents 26 (2005) 343].International Journal of Antimicrobial Agents,27(2), 181. doi:10.1016/j.ijantimicag.2005.12.002 4. Feio, S. S., Gigante, B., Roseiro, J. C., & Marcelo-Curto, M. (1999). Antimicrobial activity of diterpene resin acid derivatives.Journal of Microbiological Methods,35(3), 201-206. doi:10.1016/s0167-7012(98)001171 5. Wagh, V. D. (2013). Propolis: A Wonder Bees Product and Its Pharmacological Potentials. Advances in Pharmacological Sciences, 2013, 308249.http://doi.org/10.1155/2013/308249 Special thanks to Dr. Monika Oli, Adam Ellington, and the Department of Microbiology and Cell Science (MCB3020L) for supporting this project.