Citation
Defining The Tropism Of Various Raav Serotypes Within The Brain Ventricular System

Material Information

Title:
Defining The Tropism Of Various Raav Serotypes Within The Brain Ventricular System
Series Title:
19th Annual Undergraduate Research Symposium
Creator:
Rostkowski, Lauren
Language:
English
Physical Description:
Undetermined

Subjects

Subjects / Keywords:
Center for Undergraduate Research
Center for Undergraduate Research
Genre:
Conference papers and proceedings
Poster

Notes

Abstract:
The brain ventricular system consists of four interconnected cavities (ventricles) in the brain. They contain specialized ependymal cells involved in the production of cerebrospinal fluid (CSF). Targeting the ependymal cells lining the ventricles is an attractive target for gene therapies producing a secreted protein. We have utilized four different capsid serotypes of rAAV (1,5,6, and 8) along with four different promoters: CBA, GFAP, BLBP, and Nestin to generate 16 rAAV vectors that all express EGFP. We focused on rAAV8 because of its success in transduction of the mouse neonatal brain after the p0 injection. We observed rAAV tropism within the lateral ventricles by detecting EGFP expression using immunohistochemistry after 15 days of rAAV intraventricular injection into a neonatal mouse (p0). After 15 days, we found that rAAV tropism was most apparent with rAAV8 using the CBA promoter. The ventricle’s cells were heavily stained and distinguished from the other tissues of the brain. To validate that the virus is transducing the radial glia, we’ve decided to continue the study through a colocalization experiment using antibody cell markers. ( en )
General Note:
Research authors: Lauren Rostkowski, Pedro E. Cruz, Sanjana Bhargava, Raquel Gil, Amanda Hernandez, Sana Mahmood, Catalina Mejia, Kimberly Menezes, Odinaka Osigwe, Ramona Parkash, Neal Patel, Gia Paterno, Abraham Quader, Ankitha Reddy, Georgia Soares, Dan Tran, Trung Van, Todd E. Golde - University of Florida
General Note:
Faculty Mentor: The brain ventricular system consists of four interconnected cavities (ventricles) in the brain. They contain specialized ependymal cells involved in the production of cerebrospinal fluid (CSF). Targeting the ependymal cells lining the ventricles is an attractive target for gene therapies producing a secreted protein. We have utilized four different capsid serotypes of rAAV (1,5,6, and 8) along with four different promoters: CBA, GFAP, BLBP, and Nestin to generate 16 rAAV vectors that all express EGFP. We focused on rAAV8 because of its success in transduction of the mouse neonatal brain after the p0 injection. We observed rAAV tropism within the lateral ventricles by detecting EGFP expression using immunohistochemistry after 15 days of rAAV intraventricular injection into a neonatal mouse (p0). After 15 days, we found that rAAV tropism was most apparent with rAAV8 using the CBA promoter. The ventricle’s cells were heavily stained and distinguished from the other tissues of the brain. To validate that the virus is transducing the radial glia, we’ve decided to continue the study through a colocalization experiment using antibody cell markers. - Center for Undergraduate Research,

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University of Florida
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Copyright Lauren Rostkowski. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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