Citation
Reduction Of Planktonic And Biofilm Bacteria In Pig Skin Explant Model By Topical Lae Formulation

Material Information

Title:
Reduction Of Planktonic And Biofilm Bacteria In Pig Skin Explant Model By Topical Lae Formulation
Series Title:
19th Annual Undergraduate Research Symposium
Creator:
Rosel, Giselle
Contreras, Joyce
Language:
English
Physical Description:
Undetermined

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Subjects / Keywords:
Center for Undergraduate Research
Center for Undergraduate Research
Genre:
Conference papers and proceedings
Poster

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Abstract:
Evaluated the effects of novel formulations containing lauryl arginate ethyl ester (LAE) on killing planktonic bacteria and mature biofilms of bacteria grown on an ex-vivo model of chronic skin wounds. Sterile porcine skin explants with deep partial thickness wounds were inoculated with 50ml of Pseudomonas aeruginosa (PA01) or Staphylococcus aureus (SA113) suspension culture containing 4x 106 CFU then incubated at 37°C for 30 min followed by exposure to one of four treatments (PBS, Vehicle gel, 5% LAE gel and 1% LAE gel). Total bacterial CFU measured after 24 and 48 hours of incubation at 37°C. Inoculated explants were cultured for 3 days at 37°C to develop mature biofilms then wiped with sterile gauze and exposed to the same four test treatments, and total biofilm bacterial CFU were measured after 1, 2, and 3 days of exposure. The 1% LAE reduced the levels of total PAO1 bacteria about 2-logs by day three, while completely killing the biofilms from the first day. The 5% LAE formulation totally kills planktonic and biofilm of PA01 and SA113 bacteria on day two and three, while the 1% LAE formulation is less effective. ( en )
General Note:
Research authors: Xinxin Zhu, Hongen Zhang, Qingping Yang, Daniel Gibson and Gregory Schultz - University of Florida
General Note:
Faculty Mentor: Evaluated the effects of novel formulations containing lauryl arginate ethyl ester (LAE) on killing planktonic bacteria and mature biofilms of bacteria grown on an ex-vivo model of chronic skin wounds. Sterile porcine skin explants with deep partial thickness wounds were inoculated with 50ml of Pseudomonas aeruginosa (PA01) or Staphylococcus aureus (SA113) suspension culture containing 4x 106 CFU then incubated at 37°C for 30 min followed by exposure to one of four treatments (PBS, Vehicle gel, 5% LAE gel and 1% LAE gel). Total bacterial CFU measured after 24 and 48 hours of incubation at 37°C. Inoculated explants were cultured for 3 days at 37°C to develop mature biofilms then wiped with sterile gauze and exposed to the same four test treatments, and total biofilm bacterial CFU were measured after 1, 2, and 3 days of exposure. The 1% LAE reduced the levels of total PAO1 bacteria about 2-logs by day three, while completely killing the biofilms from the first day. The 5% LAE formulation totally kills planktonic and biofilm of PA01 and SA113 bacteria on day two and three, while the 1% LAE formulation is less effective. - Center for Undergraduate Research,

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University of Florida
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Copyright Giselle Rosel. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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! Bacterial biofilms have been found in over 80 % of chronic wounds and recent consensus guidelines recommend assuming biofilms are present in all chronic wounds 1 Bacterial biofilm are typically found beneath the surface of chronic wounds and on the surface of wound beds 2 We evaluated the effects of 1 % and 5 % formulations containing lauryl arginate ethyl ester (LAE) on killing planktonic bacteria and mature biofilms of bacteria grown on an ex vivo model of chronic skin wounds 1 % and 5% LAE could prevent biofilm formation and planktonic bacteria. 5% LAE formulation completely killed PA01 and SA planktonic bacteria on pig skin explants. Compared to the PBS treatment, the 1% LAE of both PA01 and SA bacteria only reduced the levels ~2 logs after 1, 2 and 3 days. 5% and 1% LAE completely killed the biofilm of PA01 bacteria since the 1 st day of treatment. 5 % LAE formulation reduced the biofilms of SA 113 ~ 2 logs after the 1 st day and completely killed the biofilms on the 2 nd and 3 rd days After the 1 st day 1 % LAE formulation reduced the biofilms of SA 113 ~ 1 log and completely killed them on the 2 nd and 3 rd days Sterile porcine skin explants with deep partial thickness wounds were inoculated with 50ul of Pseudomonas aeruginosa (PA01) or Staphylococcus aureus (SA113) suspension culture containing 4 x 106 CFU then incubated at 37 ¡ C for 30 min followed by exposure to one of four treatments ( PBS, Vehicle gel, 5% LAE gel and 1% LAE gel ). Total bacterial CFU was measured after 24 and 48 hours of incubation at 37 ¡ C. Inoculated explants were cultured for 3 days at 37 ¡ C to develop mature biofilms then wiped with sterile gauze and exposed to the same four treatments. Total biofilm bacterial CFU were measured after 1, 2, and 3 days of exposure. The 5% LAE formulation completely kills both planktonic and biofilm of PA01 and SA113 bacteria, while the 1% LAE formulation is less effective. 1. G. Schultz et al.. Consensus guidelines for the identification and treatment of biofilms in chronic non healing wounds, Wound Rep Regen, in press. 2. M. Fazli et al. J Clin Microbiol Non random distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds47:4084 9, 2009