Citation
Exosomes And Hypertension: How The Contents Of Exosomes Ultimately Affect Enac Activity

Material Information

Title:
Exosomes And Hypertension: How The Contents Of Exosomes Ultimately Affect Enac Activity
Series Title:
19th Annual Undergraduate Research Symposium
Creator:
Ramsay, Hunter
Language:
English
Physical Description:
Undetermined

Subjects

Subjects / Keywords:
Center for Undergraduate Research
Center for Undergraduate Research
Genre:
Conference papers and proceedings
Poster

Notes

Abstract:
Urinary exosomes are nano-sized vesicles secreted from kidney cells. They contain biomarkers and signaling molecules useful in identifying kidney associated diseases like salt-sensitive hypertension and the mechanisms of their pathogenesis. We hypothesized that dietary sodium augments the production and content of urinary exosomes to negatively regulate calcium influx and positively regulate sodium reabsorption in the aldosterone sensitive distal nephron. First, we isolated and characterized exosomes from two groups of mice, maintained on either a high salt (HS) or normal salt (NS) diet. Since decreasing intracellular calcium upregulates epithelial sodium channel (ENaC) activity, we investigated how these exosomes affect calcium influx. We loaded mouse cortical collecting duct cells (mpkCCD) with the calcium reporter dye, Cal520AM, and challenged them with either HS or NS exosomes. HS exosomes suppressed calcium influx relative to NS exosomes. Later, we discovered HS exosomes contain increased sphingomyelins over NS exosomes and that introducing exogenous sphingomyelins to mpkCCD cells mimicked the effect of HS exosomes. Future directions aim to investigate which calcium influx mechanism these sphingomyelins affect, extending the understanding of salt-sensitive hypertension and potentially leading to novel therapeutics. ( en )
General Note:
Research authors: a. Hunter Ramsay, Ling Yu, Kubra Tuna, and Abdel Alli - University of Florida
General Note:
University Scholars Program
General Note:
Faculty Mentor: Urinary exosomes are nano-sized vesicles secreted from kidney cells. They contain biomarkers and signaling molecules useful in identifying kidney associated diseases like salt-sensitive hypertension and the mechanisms of their pathogenesis. We hypothesized that dietary sodium augments the production and content of urinary exosomes to negatively regulate calcium influx and positively regulate sodium reabsorption in the aldosterone sensitive distal nephron. First, we isolated and characterized exosomes from two groups of mice, maintained on either a high salt (HS) or normal salt (NS) diet. Since decreasing intracellular calcium upregulates epithelial sodium channel (ENaC) activity, we investigated how these exosomes affect calcium influx. We loaded mouse cortical collecting duct cells (mpkCCD) with the calcium reporter dye, Cal520AM, and challenged them with either HS or NS exosomes. HS exosomes suppressed calcium influx relative to NS exosomes. Later, we discovered HS exosomes contain increased sphingomyelins over NS exosomes and that introducing exogenous sphingomyelins to mpkCCD cells mimicked the effect of HS exosomes. Future directions aim to investigate which calcium influx mechanism these sphingomyelins affect, extending the understanding of salt-sensitive hypertension and potentially leading to novel therapeutics. - Center for Undergraduate Research, University Scholars Program

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Source Institution:
University of Florida
Rights Management:
Copyright Hunter Ramsay. Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

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