Laboratory and field tests of additional organic compounds against the European corn borer

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Material Information

Title:
Laboratory and field tests of additional organic compounds against the European corn borer
Physical Description:
13 p. : ; 27 cm.
Language:
English
Creator:
Questel, D. D ( David Dewitt ), 1899-
Gertler, S. I
United States -- Bureau of Entomology and Plant Quarantine
Publisher:
U.S. Dept. of Agriculture, Agricultural Research Administration, Bureau of Entomology and Plant Quarantine
Place of Publication:
Washington, D.C
Publication Date:

Subjects

Subjects / Keywords:
European corn borer -- Control   ( lcsh )
Biological insecticides -- Testing   ( lcsh )
Genre:
federal government publication   ( marcgt )
non-fiction   ( marcgt )

Notes

General Note:
Caption title.
General Note:
"E-748."
General Note:
"May 1948."
Statement of Responsibility:
by D.D. Questel and S.I. Gertler.

Record Information

Source Institution:
University of Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 030297969
oclc - 780429515
System ID:
AA00025207:00001

Full Text

May 1948 E-748


United States Department of Agriculture
Agricultural Research Administration
Bureau of kntomolog7 and Plant Quarantine


LABOWORAT AND IBLD TESTS OF ADDITIONAL ORGANIC
COMPOUNDS AGAINST THE ZDOPZAN CORN BORER

By D. D. iestel, Division of Cereal and Forage Insect Investigations,
and. S. I. Gertler, Division of Insecticide Investigations

Laboratory investigations of organic compounds to establish their
toxicities to newly hatched larvae of the lhropean corn borer (Pyrasta
nabilalis (Hbn.)) were begun at Toledo, Ohio, early in 1938. Tests with
some of the more promising of these compounds have been conducted in the
field.* This report contains the results of laboratory tests from 1938 to
the present on compounds not previously reported in publications Z-557,
3-612, "-20, and 3.-707 of this Bureau, and field tests for the years
1945, 1946, and 19T7. All the compounds were supplied by the Division of
Insecticide Investigations,

Laboratory Tests

The care and handling of moths, eggs, and larvae for these tests and
the technique employed in conducting them are described in 0-557* Materi-
als were tested as spraWs on fresh green cauliflower or corn leaves at the
rate of 4 pounds of the compound per 100 gallons of water containing 1/3
pound of Areskep (sodiua monosulfonate of butylphenvlphenol) as a wetting
agent* Many of these compounds had. been mixed with equal parts of kaolin.
These mixtures were evaluated at an application rate twice as great as
that of the compounds which were not mixed with clay in order for the tests
to be comparable* Materials showing high mortality with little or no feed-
ing were retested at 2 pounds and 1 pound per 100 gallons of water. Larvae
in all tests were given an opportunity to feed for 48 hours before mortality
readings were taken, Results of these tests are given in tables 1, 2, and
3.
Of the 79 compounds tested at the rate of 4 pounds per 100 gallons
of water, 3 gave 100 percent kill and 2 others gave mortality higher than
90 percent. Of the 3 most promising compounds, 2 continued to show 100
percent mortality when tested at 2 pounds per 100 gallons of water. At
the rate of 1 pound per 100 gallons of water 3 compounds prodneed mortali-
ties of 94 percent or higher,*






-a-

Rield. Tests

The earliest planted fields of sweet corn were selected for these
experiments. A wheelbarrow sprayer powered with a gasoline engine and.
equipped with a nozzssle producing a solid cone of spray was used. in
applying the sprays. The plants were thoroughly treated., enough spray
being applied to cause free run-off at the base of each plant. The
quantities of spray were increased as the plants grew larger. Areskap
was used as the wetting agent at the rate of 1/3 pound per 100 gallons
of water* Plots were randomized. and replicated. 4 tines for each treat-
nent. Disesections of 100 corn plants for each treatment were made at
the roasting ear stage. The results are presented in table 4,






-3-


Table 1.-Tests at 4 pounds of compound per 100 gallons of water

Number Average percent
of mortality_ Aaount
larva Tot of
Oozpoun4L Us=~ Treated treated feeding-2


1-Phenyl eniloxamoawsid
O6H5NHNHOOOOXH2

i s(d.i salicylal)ethylened iamine,
cobalt salt
(CM : HC6Hi40)2Co

5-AetlVl-l-phenylseLoxOaaazide
Z6H5NHNHGOOONHCH,

Bi e(2-bdro7-3, 5, 6-trichlorophenyl)
methane
(C60'13OH)02

2, 4-Dichlorobenzaiid.e
00H3Cl200%r1

5-Stbyl-l-phenyl semioxamazide
W5HNHGHCOCGONHC0H5

alpha-Thiocyanoacetophenone
C6&HOH2SCO

N-3eniql-e-nltrobensamied
NO2C6H0COHCHR2CH5


124



188


123


108


131


97


79


100



100


100


97.9


92.9


36.3


18.6


114.8


0.6



1.8


.6


2.0


1.2


.5


0


+4+





+


4/ approximately the saIe
check.

gf0 0 none; + u little;


number of larvae were used in the nontreated


4+ = moderate; +++ 3 Bach.







able 1.- Contim2d


uaber Awerage percent
of mortlity Amount
larvae Not of
Compo__ usW Treated treated feeding-

2, 21, 5 I-Trichlorobenanilld5
C64CON0HC6H3012 56 14.3 0.7 -4

2-Chloro-p-bensaui side
C64CI10ONH06H4OCH3 69 13.4 .7

p.-Nitrobensoic acid,
244, 6-trichlorophenyl e*ter
N02C6HrC'00C6E2HC13 115 6.s .9 444

a-OMhloro-o-bensani side
%Hc1^cNHoir cH3c 81 6.6 ,7 44+1

2, 4-Di cloro-l-seth]lbensamide
C6HClOOHJI, 118 5.5 0 +4.4.

2,2 -lDc ohlorobensanillde
0694H010oiao4C1 130 503 06 ++

N-A1l-o-oblorobenzsld1e
06E140100mo051h 105 149 0

, N-Dibenl-o- O6E401CON(ON( O6)2 87 24. .7 +

I-amtyl-pp-oblo rob 101id04 3.9 *9 ++
0640100O0 1 0 9

SmChloro-14 '-nItwo'buisn1ld~e
2oft ^loro HS1--ni t~ b ns i 94 3-4 07 +++1
06N140100n16314302 91 3.1 .7

p-litrobensolo acid, ptolyl ester
0O646 0000614q3, 93 3.2 1.0 44+

N.,Nibeniyl.m-nltr'obensuilde
B302061400(01061)2 111 2.9 0 +++







Table 1.--OoUti=A


uimb or vergeg percent
of *-.^*'^.^ _Awnt
larvat of ft
Co-np d used Tr e treated feedio
.ohloro~la'qoloh~zql'bgasxi.d
OB06H11100HB 155 2.8 0.4 +++

2, 5 t'-Dchloro-4-nitrobensanilid.
o0^2 OOGlUmOCC1 n115 2.7 '9 +++

o2oo 3IDhlobsaulUl 162 2.5 .4 +


I, Bil bnwl-.uaitrobensamid
SOOO(DCC6") s 2.3 0 44+

h4-itrobeensallie
o264o1S 122 2.2 .9 +++
rOh1.oro-N-proWol1besasig
110NO1OOIO-iB 138 2.2 1.0 +4+

NU20 OOlMO^T -97 2.2 1.0
I-Oyclooh.el-pnitrobensamild
o1020644CON06 97 2.1 .9 ++

pe.tomo-I-,prploa.sml.
O o^0Cflo 37 88 2.1 .9++

l-w(o*hloro'bsnsyl~pip~i~tnw
C6(o=)o..io I i 96 2.1 1.0 +,+

13r1mehleroenasmIIeft
o011010 1" 2.i 0 .9 +++

3 wDidt ob.1*rob nsemids
3ingm% 109 1.9 0 -






-6-


Table l.--Continned


Number Average percent
of mortality Amount
lAre Te Not of
Compound. u960 Treated treated feedinA
l-(p-Chlorob enzoyl )-3
phenylhydrazine
C6H5HNHO0C6H1C1 i68 1.9 0o. +4+

N.- seo-Amyl-o-oblo rob enzamide
06H1C1OONiMCH(YH-)C-H7 185 1.8 i4 +44

2, 4-DI chloro-N, N-dimethkylb enzamid.e
C6H3Ci2CON(CH3)2 121i 1.7 0 +++

2..Ohloro.-3 t-nitrobensanilidAe
C6H4C100NHC6H4O2 82 1.6 .T +++

N-Bensylo--chlorob ensaide1
C6HClC0NHGCH2C6H5 12 1.6 1.0 +++

pmNitrobenzoic acid,o-tolyl ester
02C6H OOOC6H4CH3 131 1.6 1.0 +++

Bi *(dJ sail cylal) etllenediamine,
nickel salt
(CH2: CHCO6EO)4 88 1.6 .9 +4+

p-Nitrobensoic acid, p-nitrophenyl
ester
o2C6H0cooC0-H0O2 135 1.5 .9 ++

p-'hloro-N-i sobutylb enzamide
C6H(C1001MOH2CH(CH3)2 86 1.3 .9

p-MOhloro-N-ethylben zamide
C69H401NC2H5 941 1.3 411

'N-Benzyl-p-nitrobensamide
NO2C6H14COhHCH2C6H5 105 1,1 0 ..+o

1.1, 1-Trichloro-2-1m70tyl-A 17-propan
C4lC( CH 3) 2H 170O 1.1 1.7





-7-


Table 1.-- Contiinued

Number Average percent
of mortality Amount
larvae Not of
Compound usedY. Treated, treated feeding

p-Nitrobenzoic acid,
2, 4-d chlorophenyl ester
02DC6H 4COC6H3C12 107 1.1 0.9

1-(p-NI trob enzoyl )piperidine
O 2(CH2)3cX0ooc6%N0o2 102 1.0 o 0+

31 s(p-ehlorobenzyl) sulfore
(Clo6E402)2so2 94 1.0 .9 4-

2-Chloro-m-benzotoluide
06Hg lCO'ooHC6CH3 61 1.0 .7 -44

N-seo-autyl-as-.hloroben saxide
C6H0C1ONHCH(CH3)C2H5 91 1.0 0 +++

o-Chloro-N-i sopropylben &amid*
O24ClOOHCHCH 3)2 97 1.0 1.0 +o

31'-Chloro-4-nitrobenzanilide
NQ2C644C1GO1 113 .9 09
N-seo-Butyl-p-chlorobenzamide
C6H4ClOONHCH(CH3)CH5 104 .9 .9 +++

4 -.'romo-2-chlorob enzanilide
O6HRC10ONHC6H43r 91 .9 .7 ++

I-autyl-o-chlorobenzamid.e
0O6H41CONNCH9 101 .9 0 +++

p-Slitrobenzoic acid, u-tolyl eater
NO 26H4COOC6H4CH3 112 .9 .1 ++
bwaloro-'o-benzotoluide
06H4Cl00HO6HCCH3 69 .9 .7 +++





-8-


Table 1.- Continued.


Number Average percent
of mortality Amount
larva Not of
Compound uBed.I Treated, treated. feeding-
N-Benzyl-p-chlorobenzamlde
C4C1COIMCH C6H 98 0.8 0.9 +++

N-Amyl-p-chlorobenzamile
O6H4COONHC11i 122 .8 .9 +++

4-(p-NI trob enzoyl)morpholine
C2gCI0CE2CHgKOOO6H~N02 127 .8 0 4++

4-(p-Chlorobenzoyl) fmorpholine
OU^R .01 1OQ^l 40 *8 ,4 +++
C2CH20CH2CH2NOOC6ER4 1- 8.~ 4

3 4-Dichlorobenzanilide
06H4ClOONHC6H4Cl 98 .8 .9 +++

p-lorobenzyl-'p t-chlorophenyl
sulfone
GlQ QU^O^C Cl110 07. +++
0106H4CH2SO2O6HC1 no 7 .9 +T

2-Chlorobenzanilldde
C6114ClOONHC6H5 185 ,4 .4 +++

2 -Chloro-4-ni trobenzanilide
NO2C6H4CONHC6H4C1 87 0 .9+++

p-Kitrobenzoic acid,
pentachlorophenyl ester
I 2O6ECO006015 115 0 .9 +++

Ben yl-'p-chlorophe nyl sulfone
CH5CH2SOC06H4O1 105 0 .9 +++

p-Ghloroben21 pheryl sulfone
C.OC61 6i 0 0 +++

-Ch l r r1T, cy clohexylbenzamide
C6hCl OO HC6HII 88 0 .9 +++





-9-


Table 1.-- Continued


Number Average percent
of mortality Anount
larvaq Not of
Compound used& Treated treated. feedingS

MCbloro-p-benzotoluide
C C1O0N1C6BAC3 82 0.0 0.7 44+

o.Chloro-N-i sobutylb enzamide
Cl6G-1 OHOH2(CHC,3)2 4 0 0 4+

4-(o.-Chblorobenzoyl)morpholine 0 1.0
Hj2H2CH120HE OOC6H4CO 1 1.

p-1itrobensolic acid, p-tert-
butylphenyl ester
0206H)4000oo6(H 3), 89 0 ++
4 -Chloro-Jmn-itrobenzanilide
N02C6%001CONHC6H401 127 0 .9 44+

1-(p-Chlorobenzoyl )piperIdine
n(GI1)CH'POO'CH1Cl 88 0 .9 +++

2, 14-DicblorodbenIanilide
06ClOOHC6R)4Cl 73 0 .9 +++

4,404-Dichlorobenzanilide 4 44+
O6H14CIOC6OC 94 o 9 0+

l4-Chloro-2 3-nitrobenzanillde
06HR4C100lC6Oa02 75 0 09 +4+

B31 s(disali cylal) etylenedtiainne,
copper salt
(CE2rtCHo9 20t1 s0 0 09 +++

Bl8(di. alicylal) etyl en edtisine,
ferrous salt
(C21 OHC 6EO)2e 97 0 .9 ++4





-10-


Table 2.--Tests at 2 pounds of compound per 100 gallons of water

Number Average percent
of mortality Amou:.
larva7 eot of
Compound used Treated, treated feeding-2

1-Phenyl eemiozxmazide
O6H5mHCOOOH2 124 100 0.6 0

B3 8(di salicylal) etbylenediaaine,
cobalt salt
(CH: CEHC6O) 2co 199 100 1.8 +

5-.Methyl-l-phenyl semioxamazid.e
C6H5MNHHCOCONHO c, l9 97.5 .6 0 -

BI s(2-hbydroV.-3, 5, 6-tri chloro-
henyl )methane
(C6OClfO)2HCE2 158 97.2 2.0 +

5-Zthyl-l-phenyl semiozamazide
C6HIMrCOOOK CH 5 58 20.1 1.2 4+**

2, 11Dichlorobenzamiide
06H oo12COsH2 71 12.7 *5 ++.


1.2 See footnotes to table 1.





-13-


Table 3..--Tests at 1 pound of oompound per 100 gallons of water

Number Average percent
of mortality_ Aount
larvae Not of
Oopound used/ Treated treated feedi '

1-Penyl semioxamazide
C6H5NHHC00CoH 171 96.9 0.6 +

5-Methyl-l-phenyl semlioxmaazide
CCHR]fMSCOOONHCH3 143 96 .6 +

Bi s( 2-hydroay.3, 5, 6-tr, bloro-
pheny1)aethane
(C6,C.oH)2a .134 94.4 2.0 +

1 s(dis alicylal) etylenedisamine,
cobalt salt
('CK HC069o40)2Co 94 74,5 1.8 -+ M++

2, 4-.ichloroben2ide
0C6HCO12002 63 *. .5 +4+


1;. See footnotes to table 1.





-la-


Table k,--Results of field tests with some of the more promising
as indicated in laboratory trials at Toledo, Ohio


copounde


Active In- InJUr7
gredient Bleduction of to plant
per 100 bores in caused by
Compound gallons ar Plants coMpound.


Pounds
T1oo ts


Percent Peroent


DM, (technical) 25 percent
aicronized on fullerts earth

2-I eobutyryl-l-phenylbydrazlne
50 percent on kaolin

l-Pheayl-2-phenyl aulfonylhydrahine
50 percent on kaolin

a3.bis(3, 5, Dichlooro-as.lrowphenyl)
1,1-1-tri chloroethane
50 percent on pyrophyllite


1-tPhenyl eemiozamazine
50 percent on kaolin


0.5


4.0

4.0


Bis(3,5, 6-tricbloro-2-hydrozy-
phenyl) methane
50 percent on pyrophyllite

l-Phenyl-2- (p-tolyl ulfonyl)hydrazine
50 percent on kaolin


98.9 99.2


95.6 94.1


98.9 93.7


94.5 89.4

95.6 89.0


91.2 83.5

87.9 73.3


lone


Moderate


Little


Little


Moderate


Moderate

Moderate


1946 Tests


DDT, (technical) 50 percent
micronised on fuller's earth

2-.3is( 3-broao-5-ebloro--byd-roizphenyl)
1,1, l-tri chloroet bane
33 percent on pyrophyllite
2-Bis(3.5-diechloro-mhydroayphemyl)
1,1, l1-trichloreethane
50 percent on pyropkqllite
a-Bi3( 3-nitro-5r-chloro-m-hydroapheaeyl)
1,1. 1-trichloroethane
33 percent on pyrophyllite


1.0


1.33


2.0


96.7 93.8


90.7 91.0


69.3 73.4


40.0 43.3 None


lone


lone


lone


1.33






-13-


Table 4,-- Continued


Compound


Active in-
gredi ent
per 100
gallons


Reduction of
borers in
XarWe I Plants


9


a a a


Pounds Percent


Injury
to plant
ca auJp b
cmoupond


Percent


1947 Tests
1,1, l-Trichloro-2, 2-bi s(p-fluoro-
pheyl) ethane
50 percent on kaolin 2.

DDTl, (technical)
25 percent on clay

Trichlorome thyl-2, 2 -inetklyene-
bis(6-bro o4-chlorophenyl)
50 percent on clay 2.

1,1,1-Trichloro-2,2-bi s(5-chloro-.2-
bhVroyphenyl) ethane
50 percent on clay 2.

Chloromethbyl-4-chlorophezyl sulfone
50 percent on clay 2.


0


98.3


96.4



91.9



79.7


0



~0


98.2


97.2
9T-2



91.4



82.0


Little


None



None



Little


75.3 None


0 77.7





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