Laboratory tests of toxicity of some organic compounds to the European corn borer, 1940 to 1948

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Material Information

Title:
Laboratory tests of toxicity of some organic compounds to the European corn borer, 1940 to 1948
Physical Description:
11 p. : ; 27 cm.
Language:
English
Creator:
Questel, D. D ( David Dewitt ), 1899-
Gertler, S. I
United States -- Bureau of Entomology and Plant Quarantine
Publisher:
U.S. Department of Agriculture, Agricultural Research Administration, Bureau of Entomology and Plant Quarantine
Place of Publication:
Washington, D.C
Publication Date:

Subjects

Subjects / Keywords:
European corn borer -- Biological control   ( lcsh )
Biological insecticides   ( lcsh )
Genre:
federal government publication   ( marcgt )
non-fiction   ( marcgt )

Notes

Statement of Responsibility:
by D.D. Questel and S.I. Gertler.
General Note:
Caption title.
General Note:
"E-778."
General Note:
"June 1949."

Record Information

Source Institution:
University of Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 030293775
oclc - 780164410
System ID:
AA00025170:00001

Full Text



June 1949 -770


United States Department of Agriculture
Agricultural Research Administration
Bureau of Entomology and Plant Quarantine



LABORATORY TESTS OF TOXICITY OF SOME ORGAiIJC COMPOUNDS TO THE EUROPEAN CORN BORER, 1940 TO 1948


By D. D. Questel, Division of Cereal and Forage Insect Investigations,
and S. I. Gertler, Division of Insecticide Investigations



This report contains the results of laboratory tests with various organic compounds against newly hatched larvae of the European corn borer (Pyrausta nubilalis (Hbn.)) at Toledo, Ohio, during the period 1940 to 1948. Similar tests on other compounds have been reported in publications E-557, E-612, E-620, and E-707 of this Bureau. All the compounds tested were supplied by the Division of Insecticide Investigations.

The care and handling of moths, eggs, and larvae and the technique
employed in conducting these tests are described in E-557. The compounds were applied as sprays on fresh green cauliflower or corn leaves. All compounds except alpha-cyano-lN-methylacetamide had been ground with an equal weight of clay. In the initial tests either 4 or 8 pounds of the
mixture was used per 100 gallons of water containing 1/3 pound of a sodium monosulfonate of butylphenylphenol as a wetting agent. Materials showing high mortality in the initial tests were retested at lower strengths, and all larvae were given an opportunity to feed for 48 hours before mortality readings were taken. Results of these tests are given in table 1.

Technical and purified grades of 1,l,l-trichloro-2,2-bis(2-fluorophenyl)ethane gave 100 percent mortality at concentrations as low as 1/2 pound of the mixture per 100 gallons of water. The technical grade was tested in the field on sweet corn against the borer in 1947. Borer reductions of 98.2 and 98.3 percent in stalks and ears, respectively, were obtained. The percent of borer-free stalks was 94 as compared with 4 in the nontreated plots. Although some slight injury to the corn plant was observed
from the use of this compound, the plants fully recovered and there was no apparent reduction in yield.









Table i.-Toxicity to the r,3r df -,rganic compounds
tested at different.

Azyer,!,.,sent al 'roti vae Average mortality Compound Mixt-are us 4 in of I
per 100 Not
T catea)/ treated
Percent
1,1,1-Trichloro- Purified 4 OA" (0) 4
2.2-bis(P-fluoro- a (0) 4
phenvl)ethane 1 (0) 4
I 4
(C6H4F)2CHCCl3
i4 owel

Technical 4
2

1/2 (0) 4
14

o-Chlorobanzoic acid 2- (o) 2
phonylbydrazide
C6R5NMWC6R4Gl

L .-Chloro-NN-diisopropyl- 4
benzamide 2
Glc6H4CON(GH(GH3)2)2 1 (2-3)

20 4-Di chloro-4 2
nitrobenzanilide 2
Gj2G6"3CO'MO6H4NO2 a 2

traa*--ajpb.e,4,41- 4 0
Trichlorostilbene 2 0
GlC6H4Ccl:CHG6H4Cl 1 0
bet&-ftnene-carbon 9 (2) 0
totracbloride (49% 01) 4 0

2.4-Dichloro-S. 8 56 *T
isopropylbensamide 4 2 0
03- 2G6H3GONHG"( CH-2) 0 2 2 T
l/ The concentration of tLe c one-.--half the concentration
shown.
There was much feedin,- e ss t("-., vise by figures in
parentheses: (0) none; (1) little; (-") much.
2/ Approximately the same ci ..,.x-, ae used in the nontreated
checks.








Table l.-(Continued)

Zzperimental Larvae Average mortality
Compound mixture used in of larvae
per 100, t r e a x Not
Gallons men U4 1 Treated treated
Pounds Number Percent Percent
g-4,41-Tetrachlorobi- 4 232 51 (2) 0
ben2yl 1 124 0 0
ClC6H4GHclcHclC6H4Cl
3.4-Dichlorobenzoic acid 9 139 35 (2-3) .9
2--phenylbydrazide
005MMCOC03012
2.4-Dichloro-N.No-diisopropyl- 9 99 31 (0-3) .9
benzamide 4 75 24 (2) .9
ci 2116H3 OON(GR(CE 3 )2)2 2 96 2 .9

293'.4-Trichlorobanzanllide 9 01 22 (1-3) 2
C12003CONHC001 4 9 0 2
2 49 2 2
2 4,41-Trichlorobenzanilids 8 97 22 (1-3) 2
cl AH3 OONHC6"4Gl 4 3 2 2
2 0 2
44-Dichlorobenzoic acid 8 147 21
2-phenylbydrazide G6H5 GOV3012
44-Dichloro-B. 102 16 .7
propylbentaxide 4 6 .7
Gl2C6R3OONHC3H7 2 6 .7
ci e-alpba, 4, 4 I-TrI chloro- 4 155 16 (3-3) 0
istilbene,
GlG6H4CCl:C"c6H4CI
4-Dichloro-S-*tbylbensamide
cl2G6R3oONHc2H5 9 105 12 .8
4-Chlorco-31-nitro- 8 93 9 (2) .9
benzaAilide
G106400HH06%M2
2 4-Dithiobiuret 8 166 9 0
IiH2CMWC&M2








Table 1,-(Lontlaued)

Up a rimental Larvae Average mortality
0(impound mixture used in of lftaao
per 100 treat- Not
gallons!/ ment V Troat*d2/ treated Pounds Number Percent Percent
g-alpha, rapha, alpha 1-4041- a 159 9 -0
Pont achlorobiben syl 4 82 2 0
ClC6H4CHClOCI2C6R4Cl 2 105 0 0
2,4-we'riombentoylpiperldine, 8 146 9 .8
GH (CE2)1. NGDH 012
4 3
Benzoic acid, o-benzamido- 9 64 6 .8
Phenyl, ester
C05"'(' b '140OCC6H5
3.4-DIc.,Joro-NN-dIi*opropy1- 4 146 6 4
benzamide
C12C6113 00!j ( CH ( CH 3)2)2
S-Buty 1 c hlo rob an zani de 4 188 6 4
Cl2C6H3 Ouluic E
2,21,4-TrichlorobenzaAllide 8 145 5 .8
C12003(X'T- 6E4C1
4-Chl o ro- -o- .',.) on vo t olaidide 122 5 .9
01CO4 'T CH 3

2# 4-Di -ao (-iohexyl- 66 4
ban z aml, do
w I .
C12"6- i ")' ",61111
O-Bromo--: 4--dlchlorobenzi aii tl, e
C1 2CO3 go 4
N-Am j-), 4- J c-h1orobensmide 9 132 4 .8
C12C6ES'- J-' ;H 11
it-Chlorobonzulc acid, pent&- 4 64 4 0
chloroph*3z-*l eo;tar
CIC6114LkYO 0 (:1
89 4
No ;H3) 2) 2






5

Table l.-(Continued)

Experi.
mental Larvae Average mortality
Compound mixture used in of larvae
per 100 treat- Not
_[gallon;V Mont VI TreatwO/ treated Pounds Number Percent Percent
4,41-Dichlorostilbene 9 lT8 3 0
C106H4CH: CHG6H4CI
7waaric acidv bis(L-&loro- 8 157 3 0
phenyl)ester
ClG6H4OOOCH*'--HCOOG6H4Cl
2194*5t-Trichlorobenzanilide 4 150 3
ClG6"4OONHC6H3Ol2
3,4-Dichloro- -propylbenzamide 4 159 3 4
01206H 3 OOSHC 3"7 2 107 19 0

26(4-Oblorobenzamids)pyridine, 4 79 3 0
GlC6H40ONHC(CH)4N

204-Dichloro-s-'benzotoluidide 9 95 2 2
0l2C6H3ODHIiG-6H4CH3 2 143 .5 0
41-Bromo-A-chlorobenzanilide 4 86 2 0
0lG6H4GMC6Vr
41-Bromo-3.4--dichlorobenzanilide
Cl2C6H3OONHC6H4Br 8 178 2 -5
4,41-Dichlorobenzoin 4 164 2 0
CiG04CHORCH00644c'
N-Dityl-2.4-dichlorobenzamide 9 142 2 -7
01AH3001THC09
N-see-AWl-3,4-dichloro- 9 153 2 .8
benzamide
C1 2C6H3cONH CH( CH3 ) CW3H 17

3.4-Dichloro-N-isobutyl. 4 143 2 4
bensamide
ci H CONHOHOCH( C"3)2
A 3 G.








Uble 1.-(OOntinUe&)

fterimental Larvae Average mortality
Compound mixture used in of I wvas
per 100-,,treatl/ Not
gallons,-I xent Treated)/I treated
Pounds lumber Percent Percent
4 I-Bensoyi-4-eblo robenzenilide 4 142 2 0
01G644GONRC040006H5
L-Oh1oro,-N-hspt7lbsaz&uId* 4 144 2 0
01C6#4GOM'c7Rl5
3.4-Dichloro-IFN-diaetbyl- 8 149 2 0
benzamide
012C6H3 CON(GH 3)2
3.4-Dichloro-3-isopropyl- 4 166 2 4
benzouide
C1206H 3GONHOH(GE3)2
4-Ch:Loro-41-p'.henyl&zobenzanilid* 4 75 2
010644CONHOAN:N06H5
ol-Wdro:qbenzanilide 4 71 2
06H5 CONHO640H
betap-Pinens-Rexachloroethane 181 2 0
(59% 01)
2-Chloro-21-aitrobenzanilids 99 2 -7
GlG6H40ONH0CL4)M2
vwChloro-l-,* : ,propylbenzamide 4 60 1 0
OIC04001&vH('-H3)2
3-(4.0b.lorophenyl)-3 -(I -chloro- 4 159 1 0
pherq1) aoxalin
M:G
n t
IrWoro-4-isopropylbenzamide 4 60 1
OlO6R4CONHcH(cH3) 2
S-,&q1-?.,4-dicbIorobenzamid9 16o I
02206YOM'05#11








Table l.-(Coatinued)

Zxperimental Larvae Average mortality
Compound mixture used in of larvae
per 100 treat Not
I gallonsgzeni / Treate,&2/. treated
Pounds V=ber Percent Percent
3.4-Dichlorobansoic act(Igo- 9 96 1 0.8
nitrophenyl ester 012003 000"644102

3.4-Dichloro-o-bonsotoluide 9 94 1
01206"300Md6H4OH3
4 21,4,51-Tetrachlorobenzauilide 9 89 1 2
01906YONH003012
29-Brono-?-,I -diablorobonsanilide 8 97 1 2
cl 2C03 OONHG6H4Br

N-BSUZY1-3.4-dichlorobenzauide 9 173 1
01206H30ONHM2V5
1-900-ButYl-3.4-clichloro- 4 162 1 4
benzamide
GONHGH(c"3,H
MAH3 U 5)GR3
2.4-Dichloro-31-nitro- 93 1 2
benzanilide
Gl9G6H3GONHOGH4NO2
3.4-Dichloro-41-nitro- 185 1 5
benzanili(ie 012GO300NEG0002
3.4-Dichloro-31-nitro- 198 1 *5
benzanilide
"l2"030OM"04M2
alpha-47ano-N-setbylaoetamide 4 / 106 1 .8
NOCK 2 COME 3
N-Benal-21 4-dicblorobensamide 104 .9 Is
Gl2%H30ONHCH2C6H5

Not mixed with clay.








Table 1,,-(Continued)


mental 'Larvae Average mortality
Compound mixture tsed in[-- of larvae
,per 100 Itreat- No
igallons.1/pent .2-j Treatedl/ treated Pounds Number Percent Percent

2.4-Dichloro-21-nitro- 9 105 0.9 2
benzanillds
C120 6"3 CONHc6H4NO2
4-Chloro-s-benzotoluidide 4 95 -7
GlG6H4CUNHH4GF-3
21.3.4-Trichlorobenzantlide 8 156 .7
C1206H3OONHC6H4G1
3.4-Dichlaro-N-cyclobsxyl- 9 144 .5
benzamide
Gl2P6K3C'OHXc6RI1
3,4-Dichlorobenzoylaorpholine 9 172 .5
(CH2) 20(Glip) 24GOG03012
it N-DibGnzYl--3,4-di chloro- 9 161 -5
benzamide
01206H30ON(CH2005)2

2 4-Di cbI*ro-I&*2-bi cyclo- 9 189 -5 0
hevlbenzamide
06H1,106HUNG006H3012
2.4-Dichloro-l-isobntyl- 9 17 5 7
bensamide
012C6H3OONHGH2CH(GH3)2
21-Bromo-3,4.Uchloro- 9 207 .5 *5
benzanilide
ci 2 G6E3OONHC6H4Br
2,4.Dichlorobansoic acid. o- 9 196 -5 0
nitrophenyl eater
G12c;6'H3000G6H4)TO2
?-,4-Dichlorobensoic aciJ, 9 200 5 0
pent achlo rophenyl ester
C1206H3COOG6015








Table l.-(Continued)

IVerimental Larvae Lverage mortality
Compound mixture used in of larvae
per 100 treat- Not
gallons ent / Treated2/i treated
Pounds Number Percent Percent
4,41-Dichlorobenzil 4 194 0-5 0
0106HOOGOV01
2.4-Dichlorobenzoic acid, 0- 9 192 .4 0
chlorophenyl eater
012003000%401
44-Dichlor benzanisidiAs 9 199 4 0
cl NUX-H OCH
2C6"3" 6 4 3
44-Dicb1oro-o-benzaaisidide 9 192 0 0
01206H 300N"06H40CE3
44-Dichloro-2-benzotoluidide 9 197 0 0
Cl2G6R3CONH'D6H4CH3

3.4-Dichloro-21-nitro- 9 257 0 .5
benzanilide
cl 2 G 6R3(*HHC6H4102
Soso-4,41-Tetrachlorobibenz.vl 4 159 0 0
C1C6H4CHC1CHClG6H40l
3.4-Dichlorobenzoic acid,, 9 209 0 -5
pentachlorophen.vl eater
012C03COOM1 5
3,4-Dichlorobenzole acid* 0- 9 85 0 .9
chlorophen.yl ester
Gl2C6H3OOOC6H4Cl

3. 4--Di chloro-L-bon zani si dide 9 100 0 .9
Cl2c6H3GONHC6H40CH3
N- seo-autyl-2., 4-di, chloro- 9 149 0 .7
benzamide
Gle6H3cONHCH(






-10
TOU I.-Oontinued)

Zxperimental Larvae Awerage mortality
Compound mixtu" used in of larvae
per 100 trealp Not
.jjj!/ ated treated
Igal jt
Pow&& Nvaber Percent Percent
44-DichlorobenzorlAorpbelbw 8 144 0 O's
(OR2)20(GI[2)2ffG006Ek012

4-Chloro-At-nitrobenzanillde 4 112 0 19
GlG6H4OONHWO2
4-Chloro-L)..benzotoluidide 4 100 0 0
01GO40ONECACE3
NN-Dibenzyl-2-chlorobenzamide 14 129 0 0
GlG6H40ON(CH2G6H5)2
4-Cbloro-o--;kenzanisidIds 4 77 0 0
ClG6H4c&HC6H40CE3
4-Ghloro-z--benzanisidids 4 39 0 0
GlC6H4OONHG6H40OR3
29 39 6, 7-TetracUoro-r-, 6.7, 8- 4 48 0 0
tetrahydronsphthaquinone
GH .11H GOC(O)GOlucla10
,___gCHCJCRCJ( j -A
L-Mlorohippuric acid 4 64 0 0
GlG6H4OOIUfcR2c00R
p-Mlorobensoic aubydrido 4 65 0 0
(clc(AOO)?O
N-(k-Chlorobenzo7l)snthranilic 4 98 0 .8
acid
01004GONKC04COOR
L-Obloro-NN-diisobut benzamide 4 66 0 .8
ClG6%OON(GH2CH(OR ro
.; 2) 2
N-tec-Amyl-t-chlorobensamide 4 103 0 Is
GlG6H4GONHCH(GH-;)O;R7








Table 1..(Continued)

Zxperimental Larvae Average mortality
Compound mixture used in- of lAaae
per 100 treat- /1 Not
Igallon ; en t 2/ Treatedli treatedPounds Number Percent Percent
L7-Ghloro-N-eec-he3ql- 4 103 0 0.9
benzamide
OlG6R4OOHHcH(cH-,) C409
R:-Cbloro,-N-2-bic7clohexyl- 4 151 0 0
benzamide
06R11%HC NG006H501

3.4-DicbIoro,-NN-dii*obutyl- 4 125 0 4
benzamide
cl 206H3 OON(CRCGH(CE 3)2)2
2,14.5-Tetrabromocaproic acid 9 115 0 1
ON 3( GHBr)4GOOR

3. 3.4-Trichlorobenzanilide 9 169 0 .9
G1200300MG64401
3,,4,41-Trichlorobenzanilide 9 145 0
012003CONHC644cl
21.3.4,,51-Tetrachloro- 8 164 0
benzanilide
01206H30ONEV3012






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