Laboratory tests of toxicity of some organic compounds to the European corn borer, January-March 1944

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Material Information

Title:
Laboratory tests of toxicity of some organic compounds to the European corn borer, January-March 1944
Physical Description:
4 p. : ; 26 cm.
Language:
English
Creator:
Questel, D. D ( David Dewitt ), 1899-
Bowen, C. V
Gertler, S. I
United States -- Bureau of Entomology and Plant Quarantine
Publisher:
U.S. Department of Agriculture, Bureau of Entomology and Plant Quarantine
Place of Publication:
Washington, D.C
Publication Date:

Subjects

Subjects / Keywords:
European corn borer -- Biological control   ( lcsh )
Organic compounds   ( lcsh )
Genre:
federal government publication   ( marcgt )
non-fiction   ( marcgt )

Notes

Statement of Responsibility:
by D.D. Questel and C.V. Bowen and S.I. Gertler.
General Note:
Caption title.
General Note:
Typescript.
General Note:
"E-620."
General Note:
"May 1944."

Record Information

Source Institution:
University of Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 030292442
oclc - 780066175
System ID:
AA00025134:00001

Full Text


may 19 44 L1'R. Z" 620
STATE PLANT BOARD

United States Department of Ag-ricu.lture
Agricultural Research Administration
Bureau of Entomology and Plant quarantine


LABOELTOQR TESTS OF TOXICITY OF SO?9E ORGANIC COI1IOUGDS
TO M.B ETMOPEAN COIN BOBER, JANUAW-MAYRCH 1944

By D. D. Qaestel, Division of Cereal and. Forage Insect Investigations,
and C. V. Bowen and. S. I. Gertler, Division of Insecticide Investigations

This report contains results of laboratory tests with recently
decoded organic compounds showing their toxicity to newly hatched larvae
of the European corn border. Tests with other compounds against these
larvae ard reported in publications -557 and. -612 of this Bureau.

The care and handling of larvae, moths, and eggs for these tests
and, the technique employed in conducting them are described in an
earlier report. All tests were run for 48 hours. Cauliflower leaves
were used as the feeding medium.

Materials were tested as sprays at a strength of 4 pounds per
100 gallons of water containing 1/3 pound of Areskap (sodium monosulfo-
nate of butylphenylphenol) as a wetting agent. Those materials showing
high mortality with little or no feeding were retested at either 2 pounds
or 1 pound per 100 gallons of water, or both.

Only two compounds gave high mortality when the concentration was
reduced to 2 pounds per 100 gallons, and only one, 4,6-dinitro-o-cresol
ether, continued to give high mortality when tested at 1 pound per 100
gallons.

As this material is not ground readily by itself, various mix-
tures with talc and pyrophyllite were prepared and tested against the
borer. Concentrations as low as 1/4 pound of active agent per 100
gallons of water gave 100 percent mortality or nearly so in repeated
tests. The degree of fineness with which the material was ground in-
fluenced. the mortality. When such small insects as newly hatched
European corn borer larvae are being used for tests, the active agent
must be finely ground for ingestion and for the material to be carried
between the appressed leaves -rhere the borers feed in the field. Since
approximately 99 percent of the borers killed by derris spray treatments
in the field die when they are in the first or second instar, the neces-
sity for small particle size of the active agent can readily be seen.


'The results of these tests are given in table 1.




s 2 -


Table l.-Results of laboratory tests of some organic compounds against newly
hatched European corn borer larvae.

Larvae Average mortality
used in of larvae Amount'
treat- Not of
Compound. ment i/ Treated treated feeding */
Number Percent Percent
Tests at 4 pounds per 100 gallons
4,6-Dinitro-o-cresol ethyl ether 112 100.0 0.3 0
(12YOa RIo,)C6200215
Ithylidenebisbenzamide 227 98.1 08 0 to 9
d;30H(MCOQHC5) 2

8alicy32Adehyda 4-phenyl-3- 269 95.0 2.4 0 to
thiosemicarbazone
0564( OH)GOH: HOS10H05H
1-3enzoylthiourea 265 83.3 2A4 0 to*
C6H5OOHCSIH2
1-Phenylthiosemicarbazide 291 80.9 2.4 A

pp'-Dichlorophenyl sulfone 78 6s8.6 4.8 0 to w+

Cinnamaldehyde 11-phenyl- 201 67.8 2.5 +
3-thio semicarbazone
C6H6OH: OBOE: K:BSliB1
4-Camphor oxime 010917M0 90 440.0 2.1 +4

Acetylurea C530055CONH2 229 19.4 .8 +++
o-Veratraldehyde oxime 129 7.5 1.2 4+
(CH0)2056J0Hs2NOS
*cetonylaoetone dioxime 70 5.9 4.8
GH3G( tNOB)OB20( 3NOH)OB3
p-Ohlorobenpophenone 062H410006115 192 3.1 1.6 +++
4-(Phenylsulfonyl)morpholine 125 3.0 1.9 +++
06Bf5S02V(%)0^)(?H2)2
N.N-Dimethyl-N'-piperonylidene- 158 2.7 1.1 4+*
p-phenylenediamins
CR202'G 6H3OH: N6H4N(GH3, )2




-3-


Table 1.--ontinued.
Larvae Average mortality
used in of larvae Amount
treat- Not of
Compound. ment i/ Treated' treated feeding 2/
Number Percent Percent
tests at 4 pounds per 100 gallons Oont.
4.,4'-Dichlorobenzophanons oxime 113 2.6 .6 +++
(Q0i1Ql)2Gs1SQE
p-kethylacetophenone oxime 68 2,A 2.1 +++
OB305H40(OH)3:toH
1-Benzoylurea 06HH00U2 153 2.4 1.7 +++

I-Iaobutylcinnamid.e 158 1.9 .0 ++4
065HGHiOHGONHO4H9

NI-Benzy1idene-NoXI-dimethyl- 166 1.8 1.1 +++
p-phenylenediazine
"6H.1 N0612W4(CH( )3
Cyclopentanons ozime 81 1.6 4s8 ++#
0]12(OE2H)2OR2?NOR
2-7luraldiebyde 4-pb.vl-3- 190 1.6 1.7 +4+
thiosemicarbagone
(0) OH: 1imHOSIIHO6H5

Salicylaldcoxime 063(OH)OH0 111 .ll 2.1 t+t

1-Phenyl-3-(phsnylthiaureido)- 185 1.4 1.7 <*
2-thiourea
(O6H3!iHGSNH-)2
Sthyvlideneurea OH3j2TO 01jH 180 1.2 .7 +4..
2-Furenacrolein 4-phenyl-3-
thiosemicarbazone 175 1.1 1.7 es..
(04B30)OH:CHCH:N=HCSNHC6H5

4-(1,2-Dibromoethyl)toluene 100 1.0 .7 +4+
OH3OC6H14CH~rCH2Br

4-Phenylmorpholine 119 .8 1.9 4.14.
(C5)N(C2)20(CH2)2.
2,5-Bis(p-methylphenoxy) 172 .8 1.7 .*4
1,4-cyclohe:-anedione
CH3C6%09HCOCHCH(COO6TCH, ) COCH%




11-


- 4 -


uNIVjERSITY OF FLORIDA

3 1262 09239 1324


Table 1.--Continued


Larvae Average mortality
used in of larvae Amount
treat- Not of
Compound ment I/ Treated. treated feeding ?/


Number


Percent


Percent


Tests at 4 pounds per 100 gallons Ooant.


Benzaldehyde 4-phenyl-3-
thiosemicarbazone
C6HeGH: TICHCSNHAC6H5

N-p-Tolylcinnamamid..
C^H-l CHCONHC6H4CH3


Ginnamanilide


00H5CH:CHCOMNO6H5


157


169


189


N-o-Tolylainnamaaide 005Hi O1HCONHC6H4CH3 190


Cinnamamide


C6H5CH: CIM132


Tests at 2 pounds per 100 gallons


M46-Dinitro-o-cresol ethyl ether
(M%)2(CH3)C6H2OC2x

Ethylidenebisbenaanide CH3CH(ITHGOC6HV)2

Salicylaldehyde 4-phenyl-3-thiosemicarba-
zone CH4(0H)CH:NNHCSNHC6HS


d-Camphor oxime


110


147


CloH17NO


Cinnamaldehyde 4-phenyl-3-thiosemicarba-
zone C6HOCH: CHCH: NTNHCSNHC6rH5


1-Benzoyl thiourea


C6H5C0NHCSNH2


4-Phenylthiosemicarbazide C6H5NHCSNIflM2 77

Tests at 1 pound per
4,6-Dinitro-o-cresol ethyl ether 211
(NOx2)2(cH3)C6H0H5


100.0


93.1
14.0


6.7
1.4


1.3

1.3


100 gallons
100.0


Ethylidenebisbenzamide


CH3CH(mNOOCC6H5)2


0, none; 4*, little; t+, moderate: *+*, much.


1.7


t44


44,**


183


*3


2.5
2.5


2.1
2.5


2.5

2.5


ff to ++4*


4,4.
+44


4.44


14.1


1.5


I/ Approximately the same number of larvae were used in the nontreated tests.


0"