B. E. P. Q 499 Effective September 5, 1942
Supplement No. 6
TITLE 7--,AGRICULTURE CHAPTER III--BUAU OF ENTOMOLOGY AND PLANT QUARANTINE PART 301--DOI SKIC QUARANTINE NOTICES
JAAES BEETLE ADMINISTRATIVE INSTRUCTIONS MODIFIED
Pursuant to the authority conferred upon the Chief of the Bureau of
tmology and Plant Quarantine by 301.48-6, Chapter III, Title 7, Code
Of Feder Regulations regulation 6 of the rules and regulations supplemenal to Notice of Quarantine No. 48 on account of the Japanese beetle],
paragraph ( of 30148b Ecircular,.B,. E. P. Q. 499, issued June 9., 1939]
is rby amended effective September 5, 1942, by.the addition of the following subparagraph:
301.48b Adminti ate instructons to inspectors on the treatment
of ree products, rits, veetables, and soil, for the Japanese beetle.
TREATNT OF.SOIL ABOUT THE ROOTS OF PLANTS
(1) Treatment -of plants after digging
(6) Ethylene dichloride emulsion(i) Materials
Potassium hydroxide: C. P.
Alcohol: 190 proof ethyl alcohol
01eic acid: crystal white olein Ethylene dichloride: commercial
(ii) Formula l /
Alcohol-z.. --. .140 do.
Watr 6.0 do.
Oleic acid 17.5 do.
Ethylene dichloride 60.0 do.
An amount o commercial caustic potash containing an equivalent
wight of potaiu hydoxide a be substituted for the C. P. grade.
/ Completely denatured alcohol (190 proof) may be substituted for the ethyl alcohol (190 proof).
(iii) Precaration of d --Mix the several ingredients in the
order given in the formula. Dissolve the potassium hydrozide in the cohol and water, add the oleic acid, and stir interittely 10
minutes. Compensate for evaporation loss by the addition of alcohol and water in the ratio given in the for ula. Add the ethylene hloridea
The emulsible ethylene diloride shall have a specific gravity of about 1.070 at 25* C. (77'o, F.) and contain 60 percent by weight of ethy lune dichloride, It shell be a'clear solution that ay be readily diluted with water to form a uniform, stable, illike emulsion. The prdt should be kept in gastight containers in a cool place at a tempera e above C
(40' F.)** At lower temperatures it will separate into layers, in which case it must be warmed to roan temperature and stirred to restore it to its original and usable condition.
(iv) Caution.--Ethylene dichloride is an infl ble volatile sol
vent. It, the emulsible ethylene dichloride, and tbe etylee dichloride emulsion should be kept away from fire, heat, and open flame. They should be used with adequate ventilation and prolonged breathing of the vpo should be avoided.
(v) Season.-The treatment must be applied between October 1 and June 1.
(vi) Temperature.--The temperature of 'oth the dip and the plan balls at the time of dipping shall not be lower than 45* F.nor high-r than 75' F. At no time thereafter, during the holding period, shll th temperature of the treated plant balls be lower than 40 nor higher than 80.
(vii) Dosage.--Use at the rate of 1 gallon of the emulsible ethylene dichloride in 100 gallons of water. (For convenience in making amall quantities use 40 cubic centimeters in 1 gallon of water,) To prpare the emPulsible ethylene dichloride as a dip, add small quantities of water successively, stirring continually until a uniform, creamlike emulsion i formed. Dilute this emulsion with the rmaindr of the water, stir a few minutes to insure a uniform suspension, and pour into a trough or tark This dip must be prepared immediately before using.
(viii) Preparation of plants.-Plants with root masses or balls up to 10 inches in diameter at the narrowest dimension may be treated, either bare, wrapped, or in unglazd clay pots. if wrapped, the wrapping material must be of such a nature as not to prevent the proper penetration of the emulsion into the root mass. The plant bals sha be moist but not
(ix) Aaplication.--The size of the trough or tank (wood or metal) used for the dipping vat, and the quantity of the emulsion shall be ufficient to provide a complete covrae of all the plant balls. The plant balls or pots must be immersed for a period of 10 seconds in the dip. They
ay be treated either singly or in groups with the ball spaced approximately 1/4 inch apart in a wire basket or perforated tray, and arranged so as to permit of rapid penetration of the emulsion into all of the balls. In any case the plant balls or tray shall rest on the bottom of the tank. A sufficient quantity of freshly prepared, diluted emulsion shall be added to the dip so that the plant balls are completely covered during the imersion period. To reduce the hazard of plant injury, not more than the laer 1/2 inch of the plant stems should be immersed during the treatment. The contents of the trough shall be discarded -and the trough rinsed out 4 hours after charging and/or when the dirt and debris exceed 2 inches in depth The trough shall be located during plant treatments in a covered and veI ventilated place. On removal of balled plants from the dip they aybe allowed to drin into the tank for 1 or 2 minutes and then must be placed in compact group either on a bench with a tight bottom and side wall as high as the plant balls, or on a tight floor of a greenhouse, packing shed or other enclosed area, and surrounded by wooden side walls as high as thez plant balls. If they are placed on a dirt floor it must be wt and packed hard before using. In the case of potted plants any excess emulsion should be poured from the pot immediately after removing f'roam the dipping at. All plants must remain undistubed fo r the prescribed 48 hours during which time excessive ventilation should be avoided. A light pray of water applied to the tops of the plants during this period may be benefic-ial.
(x) Period of trcatment.--Ten-second immersion in the dip followed by a 48-hour holding perio~.
(xi) Vart :F 4h a1 ts .--The list of pl nts which have been sucsl tr eeri Eital work include 18 varites of analeas,
60 s of gecnouse plants, 48 kinds of perennials, ana 28 kids of
trees ad sh-rubs. The i t is subject to expansion and will be furnished on reque-st.
(7 CFR, 301. 8; see. 8, 39 Stat. 1165, 44 Stat. 250; 7 U.S.C. 161)
Done at Washington, this 4th day of September 1942.
P. N. ANNAND,
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UNIVERSITY OF FLORIDA T-iCE OF THE SECRETARY 3 22OSt 8
DR. & W BEER ENOMLOIST
STATE PLANT B0ARD
2 MAINSV8466 F1LA