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UNITED STATi'S DEPARTMENT OF AGRICULTURE Bureau of Entomology and Plant Quarantine Washington, D. C. LIBRARY STATE PLANT BOARD BEPQ-417 STERILIZATION OF IMUPOPTEI.' VJiFERA GAPES BY REFRIGERATION (Approved and effective November 9, 1936.) The method of sterilization herein authorized supersedes -Ind cancels the treatment authorized in BPQ-?62 and its supplements. Recent experimental work by the Bureau of Entomology and Plant Quarantine of the United States Department of Agriculture has proven that all stages of the Moditerranean fruit fly in fruit will be des- troyed if the fruit is subject,-d to the following tr-atme-nt: "Cooling until the approximate center of the fruit in the package reaches a temperature of 340 F. and holding the fruit at or below tha't tEmperature for a period of 12 days." On the basis of the evidence secured provision is m.-de for the entry, under permit and ste-rilization, of grapes of the Vinifcra type from regions in which the ,eloditerranean fruit fly ours, at the port of New York and such other northern ports as may be subsequently ap- proved, under the following conditions: (1) The grapes must be packed in tight barrels or kegs or other approved containers so constructed as to prevent th.- escape from the container pending stc.rilization, of any stages of the Tediterranea-i fruit fly, should they be present. Any broken containers whcraver found must be immediately repckd under the supervision of an inspector of the Burr ::u of Entomology and Plant Quarantine or the contents shall be immediately destroyed in a manner satisfactory tc the inspector. (2) VJithin twenty-four hours from the time cf unl.Iding, the grapes shall be delivered for treatment to an approved sterilization p.lant. To provide necessary safeguards for movement to and handling at approved sterilization plants, those concerns designated to sterilize fruit are required to file an - tion and complete a written agreement with the Eurcau of Entomology and Plant Quarantine. The Bureau will a:-pr-ve .-nly those plants which are adequately equipped to handle cand sterilize the fruit. Sterilization will be done under the cuaoervisin -f UNIVERSITY OF FLORIDA IIIIHI HI lulIIII~l HIH Ul II NIH IN 3 1262 09242 1832 2 - plan.t quarantine, inspectors of the Bureau of Entomology end Plant Quarantine. These inspectors shall at all times be given SCccCs to fruit while in process of sterilization. They will supervise the movement of the fruit from the docks to and from thl sterilization rooms. Shipments offered for entry may be allowed to leave customs custody under redelivery bond for sterilization. Final rclt-Lsu of the shipment by the Collector of Customs eand can- cellation cf the bond iill be effected after the inspector of the Bureau of Entomology and Plant Qu-rantine has notified the Collector of Customs that the required treatment ha.s been given. (3) F,..r the purpose of additional safeguards and to eliminate possible risk that might be occasioned by breakage of containers, the entry of grapes is liUited to the period from October 1 to April 15, when susceptible fruits will not be availablee for oviposition by fruit flies should any escape prior to the containers being placed in the approved steriliza- tion chambers. In .uthcrizing the entry of fruit into the United States, sterilized in accordance with the above requirements, it should be emphasized that inexactness und carelessness in applying the treatment may result in injury to-j the fruit, but, in event of resulting injury, neither the Depart- mcnt of Agriculture nor its employees will be responsible. Lee A. Strong, Chief, Bureau of Entomology and Plant Quarantine. |