Method and equipment for conducting laboratory experiments with insecticides for the control of insects that pupate in t...

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Material Information

Title:
Method and equipment for conducting laboratory experiments with insecticides for the control of insects that pupate in the soil
Physical Description:
2, 2 p. : ill. ; 27 cm.
Language:
English
Creator:
Snapp, Oliver I
United States -- Bureau of Entomology and Plant Quarantine
Publisher:
U.S. Department of Agriculture, Bureau of Entomology and Plant Quarantine
Place of Publication:
Washington, D.C
Publication Date:

Subjects

Subjects / Keywords:
Soil fumigation -- Equipment and supplies   ( lcsh )
Soil pesticides -- Testing   ( lcsh )
Plum curculio -- Larvae -- Control   ( lcsh )
Genre:
federal government publication   ( marcgt )
non-fiction   ( marcgt )

Notes

General Note:
Caption title.
General Note:
"ET-201."
General Note:
"September 1942."
Statement of Responsibility:
by Oliver I. Snapp.

Record Information

Source Institution:
University of Florida
Rights Management:
All applicable rights reserved by the source institution and holding location.
Resource Identifier:
aleph - 030350210
oclc - 781854723
System ID:
AA00023100:00001

Full Text





ET-201


United States Department of Agriculture
Agricultural Research Atministration
Bureau of Entomology and Plant Quarantine


METHOD AND EQUIFYENT FOR COXDUCTIhG LABORATORY
EXPERIMENTS WITH INSECTICIDES FOR TEE CONTROL
OF INSECTS THAT PUPATE IN THE SOIL

By Oliver I. Snapp,

Division of Fruit Insect Investigations



Laboratory experiments with soil fumigants against plum
curculio larvae and pupae in the soil are carried on at Fort Valley,
Ga., in boxes (fig. 1) made of 1-inch rough boards, with inside
measurements of 1 square yard and 6 inches deep, and equipped with
16-mesh wire cloth lids on hinges; all cracks are puttied and the
tops are lined with felt strips to prevent the escape of adult
insects. Such boxes would no doubt be satisfactory for similar
experiments with other insects. Plum curculio pupation usually
takes place within the top 3 inches of soil, and therefore a box
6 inches deep is adequate for experiments with that insect; however,
a deeper box would have to be used for insects that pupate under
field conditions at a depth in excess of 6 inches.

The boxes are filled with soil, after which 30 bottomless
I" x 4" glass tubes are inserted in the soil, to a depth of about
31 inches, across'the center of each box (fig. 2) for..the purpose
of determining the stage of development of the insects within each
box and to determine the rapidity with which the material under
test kills individual larvae or pupae. As each box holds 1 square
yard of soil, the material to be tested is applied at a rate of
a unit per square yard.

Two full-sized larvae are placed in each bottomless tube,
and 750 full-sized larvae are placed cn the soil in each box, to
make their way into the soil in a normal manner in preparation for
pupation. The tubes projecting about one-half inch above and
3 inches into the soil prevent the escape of the individuals frcm
within the tubes and the entrance into the tubes of the larvae
placed on the soil outside them. Individual tubes are removed
periodically to observe the development of the insects within each
box, so that treatments can be made after the larvae construct soil
cells in cases where the materials are being tested against larvae
in soil cells, after all individuals enter the pupal stage in cases







2 -


where the materials are being tested against pupae, or after
transformation to adults takes place in cases where the tests are
against adults before they leave the soil.
After the box has been treated, the remaining individual
tubes are removed at intervals to determine the rapidity of kill
from the material under test and for individual mortality records.
After the adults begin to emerge, each box is examined daily and
the adults are removed and recorded to determine the percentage of
mortality of the 750 individuals placed in the box outside of the
vials.







































Figure l--Boxes for experiments with insecticides against
larvae and pupae in the soil.






LIBRARY
STATE PLANT BOARD















































Figure 2.-Box equipped with 30 bottomless glass
tubes for individual specimens.





UNIVERSITY OF FLORIDA

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