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Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates

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Material Information

Title:
Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates
Physical Description:
Mixed Material
Language:
English
Creator:
Ju, Hye-Lim
Kang, Jung-Mi
Moon, Sung-Ung
Kim, Jung-Yeon
Lee, Hyeong-Woo
Lin, Khin
Sohn, Woon-Mok
Publisher:
Bio-Med Central (Malaria Journal)
Publication Date:

Notes

Abstract:
Background: Plasmodium vivax Duffy binding protein (PvDBP) plays an essential role in erythrocyte invasion and a potential asexual blood stage vaccine candidate antigen against P. vivax. The polymorphic nature of PvDBP, particularly amino terminal cysteine-rich region (PvDBPII), represents a major impediment to the successful design of a protective vaccine against vivax malaria. In this study, the genetic polymorphism and natural selection at PvDBPII among Myanmar P. vivax isolates were analysed. Methods: Fifty-four P. vivax infected blood samples collected from patients in Myanmar were used. The region flanking PvDBPII was amplified by PCR, cloned into Escherichia coli, and sequenced. The polymorphic characters and natural selection of the region were analysed using the DnaSP and MEGA4 programs. Results: Thirty-two point mutations (28 non-synonymous and four synonymous mutations) were identified in PvDBPII among the Myanmar P. vivax isolates. Sequence analyses revealed that 12 different PvDBPII haplotypes were identified in Myanmar P. vivax isolates and that the region has evolved under positive natural selection. High selective pressure preferentially acted on regions identified as B- and T-cell epitopes of PvDBPII. Recombination may also be played a role in the resulting genetic diversity of PvDBPII. Conclusions: PvDBPII of Myanmar P. vivax isolates displays a high level of genetic polymorphism and is under selective pressure. Myanmar P. vivax isolates share distinct types of PvDBPII alleles that are different from those of other geographical areas. These results will be useful for understanding the nature of the P. vivax population in Myanmar and for development of PvDBPII-based vaccine. Keywords: Plasmodium vivax, Duffy binding protein, Myanmar
General Note:
Ju et al. Malaria Journal 2012, 11:60 http://www.malariajournal.com/content/11/1/60; Pages 1-11
General Note:
doi:10.1186/1475-2875-11-60 Cite this article as: Ju et al.: Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates. Malaria Journal 2012 11:60.

Record Information

Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
All rights reserved by the source institution.
Resource Identifier:
oclc -
System ID:
AA00018757:00001

Material Information

Title:
Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates
Physical Description:
Mixed Material
Language:
English
Creator:
Ju, Hye-Lim
Kang, Jung-Mi
Moon, Sung-Ung
Kim, Jung-Yeon
Lee, Hyeong-Woo
Lin, Khin
Sohn, Woon-Mok
Publisher:
Bio-Med Central (Malaria Journal)
Publication Date:

Notes

Abstract:
Background: Plasmodium vivax Duffy binding protein (PvDBP) plays an essential role in erythrocyte invasion and a potential asexual blood stage vaccine candidate antigen against P. vivax. The polymorphic nature of PvDBP, particularly amino terminal cysteine-rich region (PvDBPII), represents a major impediment to the successful design of a protective vaccine against vivax malaria. In this study, the genetic polymorphism and natural selection at PvDBPII among Myanmar P. vivax isolates were analysed. Methods: Fifty-four P. vivax infected blood samples collected from patients in Myanmar were used. The region flanking PvDBPII was amplified by PCR, cloned into Escherichia coli, and sequenced. The polymorphic characters and natural selection of the region were analysed using the DnaSP and MEGA4 programs. Results: Thirty-two point mutations (28 non-synonymous and four synonymous mutations) were identified in PvDBPII among the Myanmar P. vivax isolates. Sequence analyses revealed that 12 different PvDBPII haplotypes were identified in Myanmar P. vivax isolates and that the region has evolved under positive natural selection. High selective pressure preferentially acted on regions identified as B- and T-cell epitopes of PvDBPII. Recombination may also be played a role in the resulting genetic diversity of PvDBPII. Conclusions: PvDBPII of Myanmar P. vivax isolates displays a high level of genetic polymorphism and is under selective pressure. Myanmar P. vivax isolates share distinct types of PvDBPII alleles that are different from those of other geographical areas. These results will be useful for understanding the nature of the P. vivax population in Myanmar and for development of PvDBPII-based vaccine. Keywords: Plasmodium vivax, Duffy binding protein, Myanmar
General Note:
Ju et al. Malaria Journal 2012, 11:60 http://www.malariajournal.com/content/11/1/60; Pages 1-11
General Note:
doi:10.1186/1475-2875-11-60 Cite this article as: Ju et al.: Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates. Malaria Journal 2012 11:60.

Record Information

Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
All rights reserved by the source institution.
Resource Identifier:
oclc -
System ID:
AA00018757:00001