Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

Material Information

Title:
Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains
Physical Description:
Mixed Material
Language:
English
Creator:
Dalton, Justin E.
Fear, Justin M.
Knott, Simon
Baker, Bruce S.
McIntyre, Lauren M.
Arbeitman, Michelle N.
Publisher:
Bio-Med Central (BMC Genomics)
Publication Date:

Notes

Abstract:
Background: Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (FruM). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. Results: By over-expressing individual FruM isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three FruM isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of FruM isoforms, including many annotated with neuronal functions. By determining the binding sites of individual FruM isoforms using SELEX we demonstrate that the distinct zinc finger domain of each FruM isoforms confers different DNA binding specificities. A genome-wide search for these binding site sequences finds that the gene sets identified as induced by over-expression of FruM isoforms in males are enriched for genes that contain the binding sites. An analysis of the chromosomal distribution of genes downstream of FruM shows that those that are induced and repressed in males are highly enriched and depleted on the X chromosome, respectively. Conclusions: This study elucidates the different regulatory and DNA binding activities of three FruM isoforms on a genome-wide scale and identifies genes regulated by these isoforms. These results add to our understanding of sex chromosome biology and further support the hypothesis that in some cell-types genes with male-biased expression are enriched on the X chromosome. Keywords: Fruitless, Sex hierarchy, Drosophila, Behavior, Genomics, RNA-seq
General Note:
Dalton et al. BMC Genomics 2013, 14:659 http://www.biomedcentral.com/1471-2164/14/659; Pages 1-15
General Note:
doi:10.1186/1471-2164-14-659 Cite this article as: Dalton et al.: Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains. BMC Genomics 2013 14:659.

Record Information

Source Institution:
University of Florida
Holding Location:
University of Florida
Rights Management:
All rights reserved by the source institution.
System ID:
AA00018712:00004


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